Brand  (β version)

PDB ID: 4DW7

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Ligands
Code Name Style Show Link
U Uridine-5'-monophosphate PoSSuM
C Cytidine-5'-monophosphate PoSSuM
SO4 Sulfate ion PoSSuM
Non-standard Residues
Code Name Show
Glycosylation
Code Name Emphasize
NAG 2-acetamido-2-deoxy-beta-D-glucopyranose
BMA Beta-D-mannopyranose
Modification
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Structure summary

Code : 4DW7   PDBj   RCSB PDB   PDBe
Header : VIRAL PROTEIN
Title : Crystal structure of an active-site mutant of the glycoprotein Erns from the pestivirus BVDV-1 in complex with a CpU dinucleotide
Release Data : 2012-05-23
Compound :
mol_id molecule chains
1 E(rns) glycoprotein A,B
fragment: N-terminal fragment
mutation: H32K
Source :
mol_id organism_scientific organism_common expression_system
1 Bovine viral diarrhea virus  (taxid:268305) BVDV Drosophila melanogaster  (taxid:7227)
strain: CP7
gene: Erns
expression_system_vector_type: plasmid
expression_system_plasmid: pMT-BIP-based
Authors : Krey, T., Bontems, F., Vonrhein, C., Vaney, M.-C., Bricogne, G., Ruemenapf, T., Rey, F.A.
Keywords : virus glycoprotein, T2 Ribonuclease, VIRAL PROTEIN
Exp. method : X-RAY DIFFRACTION ( 3.08 Å )
Citation :

Crystal Structure of the Pestivirus Envelope Glycoprotein E(rns) and Mechanistic Analysis of Its Ribonuclease Activity.

Krey, T.,Bontems, F.,Vonrhein, C.  et al.
(2012)  Structure  20 : 862 - 873

PubMed: 22579253
DOI: 10.1016/j.str.2012.03.018

Reaction

Chain : A, B
UniProt : Q96662 (POLG_BVDVC)
Reaction: EC: Evidence:
Physiological Direction:
[Serine protease NS3]
Leu is conserved at position P1 for all four cleavage sites. Alanine is found at position P1' of the NS4A-NS4B cleavage site, whereas serine is found at position P1' of the NS3-NS4A, NS4B-NS5A and NS5A-NS5B cleavage sites. 		3.4.21.113 -
-
[RNA-directed RNA polymerase]
a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1) 		2.7.7.48 PROSITE-ProRule:PRU00539
-
[Serine protease NS3]
a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'- diphosphate + H(+) + phosphate 		3.6.1.15 -
-
[Serine protease NS3]
ATP + H2O = ADP + H(+) + phosphate 		3.6.4.13 -
-
[E(rns) glycoprotein]
a ribonucleotidyl-ribonucleotide-RNA + H2O = a 3'-end 3'- phospho-ribonucleotide-RNA + a 5'-end dephospho-ribonucleoside-RNA + H(+) 		4.6.1.19 PubMed:22579253
left-to-right
[E(rns) glycoprotein]
a ribonucleotidyl-ribonucleotide-RNA = a 3'-end 2',3'- cyclophospho-ribonucleotide-RNA + a 5'-end dephospho-ribonucleoside- RNA 		- PubMed:22579253
left-to-right
[E(rns) glycoprotein]
a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + H2O = a 3'- end 3'-phospho-ribonucleotide-RNA + H(+) 		- PubMed:22579253
left-to-right