Brand  (β version)

color scheme of protein:

hetatm:

x
information
centroid:
interaction residue:

chain: Hide other chain(s)

Ligands
Code Name Link
C Cytidine-5'-monophosphate
U Uridine-5'-monophosphate
BMA Beta-D-mannose
MAN Alpha-D-mannose
NAG N-acetyl-D-glucosamine
SO4 Sulfate ion
Code : 4DW7
Header : VIRAL PROTEIN
Title : Crystal structure of an active-site mutant of the glycoprotein Erns from the pestivirus BVDV-1 in complex with a CpU dinucleotide
Release Data : 2012-05-23
Compound :
mol_id molecule chains
1 E(rns) glycoprotein A,B
fragment: N-terminal fragment
mutation: H32K
Source :
mol_id organism_scientific organism_common expression_system
1 Bovine viral diarrhea virus  (taxid:268305) BVDV Drosophila melanogaster  (taxid:7227)
strain: CP7
gene: Erns
expression_system_vector_type: plasmid
expression_system_plasmid: pMT-BIP-based
Authors : Krey, T., Bontems, F., Vonrhein, C., Vaney, M.-C., Bricogne, G., Ruemenapf, T., Rey, F.A.
Keywords : virus glycoprotein, T2 Ribonuclease, VIRAL PROTEIN
Exp. method : X-RAY DIFFRACTION ( 3.08 Å )
Citation :

Crystal Structure of the Pestivirus Envelope Glycoprotein E(rns) and Mechanistic Analysis of Its Ribonuclease Activity.

Krey, T.,Bontems, F.,Vonrhein, C.  et al.
(2012)  Structure  20 : 862 - 873

PubMed: 22579253
DOI: 10.1016/j.str.2012.03.018

Chain : A, B
UniProt : Q96662 (POLG_BVDVC)
Reaction : Leu is conserved at position P1 for all four cleavage sites. Alanine is found at position P1' of the NS4A-NS4B cleavage site, whereas serine is found at position P1' of the NS3- NS4A, NS4B-NS5A and NS5A-NS5B cleavage sites.
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
NTP + H(2)O = NDP + phosphate.
ATP + H(2)O = ADP + phosphate.