Brand  (β version)

PDB ID: 4DUX

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Ligands
Code Name Style Show Link
DMS Dimethyl sulfoxide PoSSuM
MG Magnesium ion PoSSuM
NA Sodium ion PoSSuM
0MK Beta-L-ribopyranose PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 4DUX   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : E. coli (lacZ) beta-galactosidase (N460S) in complex with L-ribose
Release Data : 2013-03-20
Compound :
mol_id molecule chains synonym
1 Beta-galactosidase A,B,C,D Beta-gal, Lactase
ec: 3.2.1.23
fragment: unp residues 10-1024
mutation: N460S
Source :
mol_id organism_scientific expression_system
1 Escherichia coli  (taxid:83333) Escherichia coli  (taxid:562)
strain: K12
gene: b0344, JW0335, lacZ
expression_system_strain: LMG194
expression_system_vector_type: plasmid
expression_system_plasmid: pBAD/HIS/LacZ
Authors : Wheatley, R.W., Lo, S., Janzcewicz, L.J., Dugdale, M.L., Huber, R.E.
Keywords : bi-functional enzyme, allolactose synthesis, TIM BARREL (ALPHA/BETA BARREL), JELLY-ROLL BARREL, IMMUNOGLOBULIN, BETA SUPERSANDWICH, BETA-GALACTOSIDASE, GLYCOSIDASE, HYDROLASE
Exp. method : X-RAY DIFFRACTION ( 2.30 Å )
Citation :

Structural explanation for allolactose (lac operon inducer) synthesis by lacZ beta-galactosidase and the evolutionary relationship between allolactose synthesis and the lac repressor.

Wheatley, R.W.,Lo, S.,Jancewicz, L.J.  et al.
(2013)  J.Biol.Chem.  288 : 12993 - 13005

PubMed: 23486479
DOI: 10.1074/jbc.M113.455436

Reaction

Chain : A, B, C, D
UniProt : P00722 (BGAL_ECOLI)
Reaction: EC: Evidence:
Physiological Direction:
Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. 3.2.1.23 -
-
Cofactor: Evidence: Note:
Mn(2+) - Binds 2 magnesium ions per monomer. Can also use manganese.
Na(+) - Binds 1 sodium ion per monomer.