Brand  (β version)

PDB ID: 4DSI

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Ligands
Code Name Style Show Link
CA Calcium ion PoSSuM
DGP 2'-deoxyguanosine-5'-monophosphate PoSSuM
GOL Glycerol PoSSuM
SO4 Sulfate ion PoSSuM
Non-standard Residues
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Structure summary

Code : 4DSI   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE/DNA
Title : Crystal structure of fragment DNA polymerase I from Bacillus stearothermophilus with duplex DNA, Se-dGTP and Calcium
Release Data : 2013-03-27
Compound :
mol_id molecule chains
1 DNA polymerase A
ec: 2.7.7.7
fragment: RESIDUES 298-876
mutation: A598D
mol_id molecule chains
2 DNA B
mol_id molecule chains
3 Se-dGTP C
Source :
mol_id organism_scientific expression_system
1 Geobacillus stearothermophilus  (taxid:1422) Escherichia coli  (taxid:562)
gene: DPO1
expression_system_strain: BL21 (DE3)
expression_system_vector_type: plasmid
expression_system_plasmid: pET28
mol_id organism_scientific
2
synthetic: yes
other_details: Synthesized DNA
mol_id organism_scientific
3
synthetic: yes
other_details: Se-modified dGTP
Authors : Gan, J.H., Abdur, R., Liu, H.H., Sheng, J., Caton-Willians, J., Soares, A.S., Huang, Z.
Keywords : DNA polymerase, polymerization fidelity, calcium cation, purine selectivity, blunt end extension, base stacking, DNA synthesis, TRANSFERASE-DNA complex
Exp. method : X-RAY DIFFRACTION ( 2.05 Å )
Citation :

Biochemical and structural insights into the fidelity of bacillus stearothermophilus DNA polymerase

Gan, J.H.,Abdur, R.,Liu, H.H.  et al.
To be Published 

Reaction

Chain : A
UniProt : D9N168 (D9N168_GEOSE)
Reaction: EC: Evidence:
Physiological Direction:
DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate 2.7.7.7 ARBA:ARBA00049244, RuleBase:RU004460
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