Brand  (β version)

color scheme of protein:

hetatm:

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information
centroid:
interaction residue:

chain: Hide other chain(s)

Ligands
Code Name Link
MG Magnesium ion
0KX 2'-deoxy-5'-O-[(R)-hydroxy{[(R)-hydroxy(phosphonooxy)phosphoryl]amino}phosphoryl]cytidine
GOL Glycerol
Code : 4DL2
Header : TRANSFERASE/DNA/INHIBITOR
Title : Human DNA polymerase eta inserting dCMPNPP opposite CG template (GG0a)
Release Data : 2012-05-09
Compound :
mol_id molecule chains synonym
1 DNA polymerase eta A RAD30 homolog A, Xeroderma pigmentosum variant type protein
ec: 2.7.7.7
fragment: unp residues 1-432
mol_id molecule chains
2 DNA (5'-D(*AP*CP*GP*GP*CP*TP*CP*AP*CP*AP*CP*T)-3') T
mol_id molecule chains
3 DNA (5'-D(*TP*AP*GP*TP*GP*TP*GP*AP*G)-3') P
other_details: dCMPNPP
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Escherichia coli  (taxid:469008)
gene: POLH, RAD30, RAD30A, XPV
expression_system_strain: BL21(DE3) plysS
expression_system_vector_type: plasmid
expression_system_plasmid: pET28
mol_id organism_scientific
2
synthetic: yes
other_details: oligonucleotide, chain T
mol_id organism_scientific
3
synthetic: yes
other_details: oligonucleotide, chain P
Authors : Zhao, Y., Biertumpfel, C., Gregory, M., Hua, Y., Hanaoka, F., Yang, W.
Keywords : cisplatin, chemoresistence, translesion synthesis, human DNA polymerse eta, kinetics, molecular splint, inhibition, DNA distorsion, second TLS polymerase, nucleotidyl transfer reaction, PCNA, TRANSFERASE-DNA-INHIBITOR complex
Exp. method : X-RAY DIFFRACTION ( 2.15 Å )
Citation :

Structural Basis for Chemoresistance to Cisplatin Mediated by DNA Polymerase eta

Zhao, Y.,Biertumpfel, C.,Gregory, M.T.  et al.
(2012)  Proc.Natl.Acad.Sci.USA  109 : 7269 - 7274

PubMed: 22529383
DOI: 10.1073/pnas.1202681109

Chain : A
UniProt : Q9Y253 (POLH_HUMAN)
Reaction : Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).