Brand  (β version)

PDB ID: 4DKJ

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Ligands
Code Name Style Show Link
GOL Glycerol PoSSuM
SAH S-adenosyl-L-homocysteine PoSSuM
Non-standard Residues
Code Name Show
5CM 5-methyl-2'-deoxy-cytidine-5'-monophosphate
C37 5-fluoro-2'-deoxy-cytidine-5'-monophosphate
Glycosylation
Code Name Emphasize
Modification
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Structure summary

Code : 4DKJ   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE/DNA
Title : CpG specific methyltransferase in complex with target DNA
Release Data : 2012-12-05
Compound :
mol_id molecule chains
1 Cytosine-specific methyltransferase A
ec: 2.1.1.37
mutation: S295P
mol_id molecule chains
2 DNA (5'-D(*CP*CP*AP*CP*AP*TP*GP*(C37)P*GP*CP*TP*GP*AP*A)-3') B
mol_id molecule chains
3 DNA (5'-D(*GP*TP*TP*CP*AP*GP*(5CM)P*GP*CP*AP*TP*GP*TP*G)-3') C
Source :
mol_id organism_scientific expression_system
1 Mycoplasma penetrans  (taxid:272633) Escherichia coli  (taxid:562)
strain: HF-2
gene: MYPE4940
expression_system_strain: ER2566
expression_system_vector_type: PLASMID
expression_system_plasmid: PET28A
mol_id organism_scientific
2
synthetic: yes
mol_id organism_scientific
3
synthetic: yes
Authors : Wojciechowski, M., Czapinska, H., Bochtler, M.
Keywords : CG-SPECIFICITY, DNA INTERCALATION, CPG SEQUENCE, CYTOSINE C5-METHYLATION, C5-METHYLCYTOSINE, NUCLEOTIDE FLIPPING, S-adenosyl-L-methionine-dependent methyltransferases, C-5 cytosine-specific DNA methylases, DNA (cytosine-5-)-methyltransferase activity, DNA binding, DNA (cytosine-5-)-methylation, intracellular, TRANSFERASE-DNA complex
Exp. method : X-RAY DIFFRACTION ( 2.15 Å )
Citation :

CpG underrepresentation and the bacterial CpG-specific DNA methyltransferase M.MpeI.

Wojciechowski, M.,Czapinska, H.,Bochtler, M.
(2013)  Proc.Natl.Acad.Sci.USA  110 : 105 - 110

PubMed: 23248272
DOI: 10.1073/pnas.1207986110

Reaction

Chain : A
UniProt : Q8EVR5 (Q8EVR5_MALP2)
Reaction: EC: Evidence:
Physiological Direction:
a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = a 5- methyl-2'-deoxycytidine in DNA + H(+) + S-adenosyl-L-homocysteine 2.1.1.37 RuleBase:RU000417
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