Brand  (β version)

PDB ID: 4DFJ

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Ligands
Code Name Style Show Link
0KL 5-(5-aminopent-1-yn-1-yl)-2'-deoxyuridine 5'-(tetrahydrogen triphosphate) PoSSuM
CL Chloride ion PoSSuM
EDO 1,2-ethanediol PoSSuM
FMT Formic acid PoSSuM
MG Magnesium ion PoSSuM
Non-standard Residues
Code Name Show
DOC 2',3'-dideoxycytidine-5'-monophosphate
Glycosylation
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Modification
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Structure summary

Code : 4DFJ   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE/DNA
Title : Crystal structure of the large fragment of DNA Polymerase I from Thermus aquaticus in a closed ternary complex with 5-(aminopentinyl)-dTTP
Release Data : 2012-05-16
Compound :
mol_id molecule chains synonym
1 DNA polymerase I, thermostable A Taq polymerase 1
ec: 2.7.7.7
fragment: Klenow Fragment
mol_id molecule chains
2 5'-d(GACCACGGCGC DOC)-3' B
fragment: DNA Primer
mol_id molecule chains
3 5'-d(AAAAGGCGCCGTGGTC)-3' C
fragment: DNA Template
Source :
mol_id organism_scientific expression_system
1 Thermus aquaticus  (taxid:271) Escherichia coli  (taxid:469008)
gene: Pol I, pol1, polA
expression_system_strain: BL21-DE3
expression_system_vector_type: pET series
expression_system_plasmid: pET21b
mol_id organism_scientific
2
synthetic: yes
other_details: DNA synthesizer
mol_id organism_scientific
3
synthetic: yes
other_details: DNA synthesizer
Authors : Bergen, K., Steck, A., Struett, S., Baccaro, A., Welte, W., Diederichs, K., Marx, A.
Keywords : DNA Polymerase, TRANSFERASE-DNA complex
Exp. method : X-RAY DIFFRACTION ( 1.9 Å )
Citation :

Structures of KlenTaq DNA Polymerase Caught While Incorporating C5-Modified Pyrimidine and C7-Modified 7-Deazapurine Nucleoside Triphosphates.

Bergen, K.,Steck, A.L.,Strutt, S.  et al.
(2012)  J.Am.Chem.Soc.  134 : 11840 - 11843

PubMed: 22475415
DOI: 10.1021/ja3017889

Reaction

Chain : A
UniProt : P19821 (DPO1_THEAQ)
Reaction: EC: Evidence:
Physiological Direction:
DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate 2.7.7.7 UniProtKB:P52026
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