Brand  (β version)

PDB ID: 4D7I

Hetero Atom Contents

  The number of atoms exceeds 100,000.
  So, it can not be displayed here.

Select unit:

Select hetatm:   

close
information
centroid:
interaction residue:

Select chain:   Sequence  

Data format:   

Color scheme of protein:

Ligands
Code Name Style Show Link
0GD 6-[4-({[2-(3-fluorophenyl)ethyl]amino}methyl)phenyl]-4-methylpyridin-2-amine PoSSuM
CL Chloride ion PoSSuM
GOL Glycerol PoSSuM
H4B 5,6,7,8-tetrahydrobiopterin PoSSuM
HEM Protoporphyrin ix containing Fe PoSSuM
Non-standard Residues
Code Name Show
Glycosylation
Code Name Emphasize
Modification
Code Name Show

Structure summary

Code : 4D7I   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : Structure of Bacillus subtilis nitric oxide synthase I218V in complex with 6-(4-(((3-Fluorophenethyl)amino)methyl)phenyl)-4-methylpyridin-2- amine
Release Data : 2015-07-01
Compound :
mol_id molecule chains synonym
1 NITRIC OXIDE SYNTHASE OXYGENASE A NOSOXY-LIKE PROTEIN, BACILLUS SUBTILIS NITRIC OXIDE SYNTHASE
ec: 1.14.13.165
mutation: YES
Source :
mol_id organism_scientific expression_system
1 BACILLUS SUBTILIS SUBSP. SUBTILIS STR. 168  (taxid:224308) ESCHERICHIA COLI  (taxid:469008)
atcc: 23857
expression_system_strain: BL21(DE3)
expression_system_vector_type: PLASMID
expression_system_vector: PET28A
Authors : Holden, J.K., Poulos, T.L.
Keywords : OXIDOREDUCTASE, NITRIC OXIDE SYNTHASE
Exp. method : X-RAY DIFFRACTION ( 1.960 Å )
Citation :

Nitric Oxide Synthase as a Target for Methicillin-Resistant Staphylococcus Aureus

Holden, J.K.,Kang, S.,Beasley, F.C.  et al.
(2015)  Chem.Biol.  22 : 785

PubMed: 26091171
DOI: 10.1016/J.CHEMBIOL.2015.05.013

Reaction

Chain : A
UniProt : O34453 (NOSO_BACSU)
Reaction: EC: Evidence:
Physiological Direction:
3 reduced [flavodoxin] + 2 L-arginine + 4 O2 = 3 oxidized [flavodoxin] + 2 L-citrulline + 2 nitric oxide + 4 H2O + 5 H(+) 1.14.14.47 PubMed:11856757
-
Cofactor: Evidence: Note:
heme ECO:0000269 | PubMed:11856757
-
(6R)-L-erythro-5,6,7,8-tetrahydrobiopterin ECO:0000269 | PubMed:11856757
Tetrahydrobiopterin (H4B). Can also use tetrahydrofolate (THF) in place of tetrahydrobiopterin (H4B) but binds THF with much lower affinity than H4B.