Brand  (β version)

PDB ID: 4CS3

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Ligands
Code Name Style Show Link
AMP Adenosine monophosphate PoSSuM
EDO 1,2-ethanediol PoSSuM
FU0 2-(furan-2-yl)ethyl hydrogen carbonate PoSSuM
LYS Lysine PoSSuM
MG Magnesium ion PoSSuM
POP Pyrophosphate 2- PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 4CS3   PDBj   RCSB PDB   PDBe
Header : LIGASE
Title : Catalytic domain of Pyrrolysyl-tRNA synthetase mutant Y306A, Y384F in complex with an adenylated furan-bearing noncanonical amino acid and pyrophosphate
Release Data : 2014-04-30
Compound :
mol_id molecule chains synonym
1 PYRROLYSINE--TRNA LIGASE A PYRROLYSINE--TRNA(PYL) LIGASE, PYRROLYSYL-TRNA SYNTHETASE, PYLRS
ec: 6.1.1.26
fragment: C-TERMINAL FRAGMENT, RESIDUES 188-454
mutation: YES
Source :
mol_id organism_scientific expression_system
1 METHANOSARCINA MAZEI  (taxid:2209) ESCHERICHIA COLI  (taxid:562)
expression_system_strain: TOP10
Authors : Schmidt, M.J., Weber, A., Pott, M., Welte, W., Summerer, D.
Keywords : LIGASE, AMINOACYL-TRNA SYNTHETASE, PYRROLYSYL-TRNA-SYNTHETASE, NONCANONICAL AMINO ACIDS, FURANS, CROSSLINKING REACTIONS
Exp. method : X-RAY DIFFRACTION ( 1.499 Å )
Citation :

Structural Basis of Furan-Amino Acid Recognition by a Polyspecific Aminoacyl-tRNA-Synthetase and its Genetic Encoding in Human Cells.

Schmidt, M.J.,Weber, A.,Pott, M.  et al.
(2014)  Chembiochem  15 : 1755

PubMed: 24737732
DOI: 10.1002/CBIC.201402006

Red-Light-Controlled Protein-RNA Crosslinking with a Genetically Encoded Furan.

Schmidt, M.J.,Summerer, D.
(2013)  Angew.Chem.Int.Ed.Engl.  52 : 4690

PubMed: 23512703
DOI: 10.1002/ANIE.201300754

Reaction

Chain : A
UniProt : Q8PWY1 (PYLS_METMA)
Reaction: EC: Evidence:
Physiological Direction:
ATP + L-pyrrolysine + tRNA(Pyl) = AMP + diphosphate + L- pyrrolysyl-tRNA(Pyl) 6.1.1.26 HAMAP- Rule:MF_01573
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