Brand  (β version)

PDB ID: 4CF6

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Ligands
Code Name Style Show Link
CBD Cibacron blue PoSSuM
FAD Flavin-adenine dinucleotide PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 4CF6   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : Crystal structure of the complex of the P187S variant of human NAD(P) H:quinone oxidoreductase with Cibacron blue at 2.7 A resolution
Release Data : 2014-08-27
Compound :
mol_id molecule chains synonym
1 NAD(P)H DEHYDROGENASE [QUINONE] 1 A,B AZOREDUCTASE, DT-DIAPHORASE, DTD, MENADIONE REDUCTASE, NAD(P)H\:QUINONE OXIDOREDUCTASE 1, PHYLLOQUINONE REDUCTASE, QUINONE REDUCTASE 1, QR1, NADPH QUINONE OXIDOREDUCTASE
ec: 1.6.5.2
mutation: YES
Source :
mol_id organism_scientific organism_common expression_system
1 HOMO SAPIENS  (taxid:9606) HUMAN ESCHERICHIA COLI  (taxid:511693)
expression_system_strain: BL21
expression_system_variant: STAR
expression_system_vector_type: PLASMID
expression_system_vector: PET28A
Authors : Lienhart, W.D., Gudipati, V., Uhl, M.K., Binter, A., Pulido, S., Saf, R., Zangger, K., Gruber, K., Macheroux, P.
Keywords : FLAVOPROTEIN, OXIDOREDUCTASE, OXIDOREDUCTASE-INHIBITOR COMPLEX, SINGLE AMINO ACID EXCHANGE
Exp. method : X-RAY DIFFRACTION ( 2.694 Å )
Citation :

Collapse of the Native Structure by a Single Amino Acid Exchange in Human Nad(P)H:Quinone Oxidoreductase (Nqo1).

Lienhart, W.D.,Gudipati, V.,Uhl, M.K.  et al.
(2014)  FEBS J.  281 : 4691

PubMed: 25143260
DOI: 10.1111/FEBS.12975

Reaction

Chain : A, B
UniProt : P15559 (NQO1_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
a quinone + H(+) + NADH = a quinol + NAD(+) 1.6.5.2 PubMed:8999809, PubMed:9271353
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a quinone + H(+) + NADPH = a quinol + NADP(+) 1.6.5.2 PubMed:9271353
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H(+) + NADH + ubiquinone-10 = NAD(+) + ubiquinol-10 - PubMed:9271353
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H(+) + menadione + NADH = menadiol + NAD(+) - PubMed:8999809
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