Brand  (β version)

PDB ID: 4C6M

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Ligands
Code Name Style Show Link
FMT Formic acid PoSSuM
FOT 5-fluoro-2,6-dioxo-1,2,3,6-tetrahydropyrimidine-4-carboxylic acid PoSSuM
ZN Zinc ion PoSSuM
Non-standard Residues
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KCX Lysine nz-carboxylic acid
Glycosylation
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Structure summary

Code : 4C6M   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : Crystal structure of the dihydroorotase domain of human CAD bound to the inhibitor fluoroorotate at pH 7.0
Release Data : 2014-01-08
Compound :
mol_id molecule chains synonym
1 CAD PROTEIN A DIHYDROOROTASE
ec: 3.5.2.3
fragment: RESIDUES 1456-1846
Source :
mol_id organism_scientific organism_common expression_system
1 HOMO SAPIENS  (taxid:9606) HUMAN HOMO SAPIENS  (taxid:9606)
expression_system_common: HUMAN
expression_system_cell_line: HEK293
expression_system_vector_type: PLASMID
expression_system_plasmid: POPIN-M
Authors : Ramon-Maiques, S., Lallous, N., Grande-Garcia, A.
Keywords : HYDROLASE, DE NOVO PYRIMIDINE BIOSYNTHESIS, AMIDOHYDROLASE SUPERFAMILY, METALLOENZYME, ZINC BINDING, HISTIDINATE ANION
Exp. method : X-RAY DIFFRACTION ( 1.620 Å )
Citation :

Structure, Functional Characterization and Evolution of the Dihydroorotase Domain of Human Cad.

Grande-Garcia, A.,Lallous, N.,Diaz-Tejada, C.  et al.
(2014)  Structure  22 : 185

PubMed: 24332717
DOI: 10.1016/J.STR.2013.10.016

Reaction

Chain : A
UniProt : P27708 (PYR1_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
2 ATP + H2O + hydrogencarbonate + L-glutamine = 2 ADP + carbamoyl phosphate + 2 H(+) + L-glutamate + phosphate 6.3.5.5 PubMed:24332717
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H2O + L-glutamine = L-glutamate + NH4(+) 3.5.1.2 UniProtKB:P07259
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2 ATP + hydrogencarbonate + NH4(+) = 2 ADP + carbamoyl phosphate + 2 H(+) + phosphate 6.3.4.16 UniProtKB:P07259
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carbamoyl phosphate + L-aspartate = H(+) + N-carbamoyl-L- aspartate + phosphate 2.1.3.2 PubMed:24332717
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(S)-dihydroorotate + H2O = H(+) + N-carbamoyl-L-aspartate 3.5.2.3 PubMed:24332717
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