Brand  (β version)

PDB ID: 4C6L

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Ligands
Code Name Style Show Link
FMT Formic acid PoSSuM
FOT 5-fluoro-2,6-dioxo-1,2,3,6-tetrahydropyrimidine-4-carboxylic acid PoSSuM
ZN Zinc ion PoSSuM
Non-standard Residues
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KCX Lysine nz-carboxylic acid
Glycosylation
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Structure summary

Code : 4C6L   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : Crystal structure of the dihydroorotase domain of human CAD bound to the inhibitor fluoroorotate at pH 6.0
Release Data : 2014-02-05
Compound :
mol_id molecule chains synonym
1 CAD PROTEIN A DIHYDROOROTASE
ec: 3.5.2.3
fragment: RESIDUES 1456-1846
Source :
mol_id organism_scientific organism_common expression_system
1 HOMO SAPIENS  (taxid:9606) HUMAN HOMO SAPIENS  (taxid:9606)
expression_system_common: HUMAN
expression_system_cell_line: HEK293
expression_system_vector_type: PLASMID
expression_system_plasmid: POPIN-M
Authors : Ramon-Maiques, S., Lallous, N., Grande-Garcia, A.
Keywords : HYDROLASE, DE NOVO PYRIMIDINE BIOSYNTHESIS, AMIDOHYDROLASE SUPERFAMILY, METALLOENZYME, ZINC BINDING, HISTIDINATE ANION
Exp. method : X-RAY DIFFRACTION ( 1.550 Å )
Citation :

Structure, Functional Characterization and Evolution of the Dihydroorotase Domain of Human Cad.

Grande-Garcia, A.,Lallous, N.,Diaz-Tejada, C.  et al.
(2014)  Structure  22 : 185

PubMed: 24332717
DOI: 10.1016/J.STR.2013.10.016

Reaction

Chain : A
UniProt : P27708 (PYR1_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
hydrogencarbonate + L-glutamine + 2 ATP + H2O = carbamoyl phosphate + L-glutamate + 2 ADP + phosphate + 2 H(+) 6.3.5.5 PubMed:24332717
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L-glutamine + H2O = L-glutamate + NH4(+) 3.5.1.2 UniProtKB:P07259
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hydrogencarbonate + NH4(+) + 2 ATP = carbamoyl phosphate + 2 ADP + phosphate + 2 H(+) 6.3.4.16 UniProtKB:P07259
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carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+) 2.1.3.2 PubMed:24332717
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(S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate + H(+) 3.5.2.3 PubMed:24332717
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Cofactor: Evidence: Note:
Zn(2+) ECO:0000269 | PubMed:24332717
ECO:0000269 | PubMed:7766613
Binds 3 Zn(2+) ions per subunit (for dihydroorotase activity).
Mn(2+) ECO:0000255 | PROSITE-ProRule:PRU00409
ECO:0000255 | PROSITE-ProRule:PRU00409
Binds 4 magnesium or manganese ions per subunit.