PDB ID: 4AH9
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|Title :||Parallel screening of a low molecular weight compound library: do differences in methodology affect hit identification|
|Release Data :||2012-12-19|
|Authors :||Wielens, J., Heady, S.J., Rhodes, D.I., Mulder, R.J., Dolezal, O., Deadman, J.J., Newman, J., Chalmers, D.K., Parker, M.W., Peat, T.S., Scanlon, M.J.|
|Keywords :||TRANSFERASE, FRAGMENT SCREENING|
|Exp. method :||X-RAY DIFFRACTION ( 1.70 Å )|
|Chain :||A, B|
|UniProt :||P12497 (POL_HV1N5)|
|Reaction :||Specific for a P1 residue that is hydrophobic, and P1' variable, but often Pro.
Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.
3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid.
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).