Brand  (β version)

PDB ID: 3WQD

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Ligands
Code Name Style Show Link
999 (3s)-3-hydroxy-D-aspartic acid PoSSuM
MG Magnesium ion PoSSuM
PLP Pyridoxal-5'-phosphate PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 3WQD   PDBj   RCSB PDB   PDBe
Header : LYASE
Title : D-threo-3-hydroxyaspartate dehydratase from Delftia sp. HT23 complexed with D-erythro-3-hydroxyaspartate
Release Data : 2015-01-28
Compound :
mol_id molecule chains synonym
1 D-threo-3-hydroxyaspartate dehydratase A,B D-THA DH, D-THA dehydratase, Threo-3-hydroxy-D-aspartate ammonia-lyase
ec: 4.3.1.27
Source :
mol_id organism_scientific expression_system
1 Delftia  (taxid:518882) Rhodococcus erythropolis  (taxid:1833)
strain: HT23
gene: dthadh
expression_system_strain: L88
expression_system_vector_type: plasmid
expression_system_plasmid: pTip-QC2
Authors : Yasutake, Y., Matsumoto, Y., Wada, M.
Keywords : dehydratase, PLP, Lyase
Exp. method : X-RAY DIFFRACTION ( 1.50 Å )
Citation :

Structural insights into the substrate stereospecificity of D-threo-3-hydroxyaspartate dehydratase from Delftia sp. HT23: a useful enzyme for the synthesis of optically pure L-threo- and D-erythro-3-hydroxyaspartate

Matsumoto, Y.,Yasutake, Y.,Takeda, Y.  et al.
(2015)  Appl.Microbiol.Biotechnol.  99 : 7137 - 7150

PubMed: 25715785
DOI: 10.1007/s00253-015-6479-3

Reaction

Chain : A, B
UniProt : B2DFG5 (DTHAD_DELSH)
Reaction: EC: Evidence:
Physiological Direction:
(3R)-3-hydroxy-D-aspartate = NH4(+) + oxaloacetate 4.3.1.27 PubMed:20843822
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