Brand  (β version)

color scheme of protein:

hetatm:

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information
centroid:
interaction residue:

chain: Hide other chain(s)

Ligands
Code Name Link
FNN 3-fluoro-4-nitrophenol
Code : 3VGN
Header : ISOMERASE
Title : Crystal Structure of Ketosteroid Isomerase D40N from Pseudomonas putida (pKSI) with bound 3-fluoro-4-nitrophenol
Release Data : 2012-08-22
Compound :
mol_id molecule chains synonym
1 Steroid Delta-isomerase A,B Delta(5)-3-ketosteroid isomerase
ec: 5.3.3.1
mutation: D40N
Source :
mol_id organism_scientific expression_system
1 Pseudomonas putida  (taxid:303) Escherichia coli  (taxid:562)
gene: ksi
expression_system_strain: BL21
expression_system_vector_type: plasmid
Authors : Caaveiro, J.M.M., Pybus, B., Ringe, D., Petsko, G.A., Sigala, P.A.
Keywords : hydrogen bond, KSI, enzyme catalysis, transition state, oxyanion hole, ISOMERASE
Exp. method : X-RAY DIFFRACTION ( 1.3000 Å )
Citation :

Quantitative dissection of hydrogen bond-mediated proton transfer in the ketosteroid isomerase active site

Sigala, P.A.,Fafarman, A.T.,Schwans, J.P.  et al.
(2013)  Proc.Natl.Acad.Sci.USA  110 : E2552 - E2561

PubMed: 23798390
DOI: 10.1073/pnas.1302191110

Chain : A, B
UniProt : P07445 (SDIS_PSEPU)
Reaction : A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)- steroid.