Brand  (β version)

PDB ID: 3VF2

Hetero Atom Contents

color scheme of protein:

hetatm:

x
information
centroid:
interaction residue:

chain:

Ligands
Code Name Link
ADP Adenosine-5'-diphosphate PoSSuM
CP Phosphoric acid mono(formamide)ester PoSSuM
FE2 Fe (II) ion PoSSuM
K Potassium ion PoSSuM

Structure summary

Code : 3VF2
Header : TRANSFERASE
Title : Crystal structure of the O-carbamoyltransferase TobZ M473I variant in complex with carbamoyl phosphate and ADP
Release Data : 2012-01-25
Compound :
mol_id molecule chains
1 O-carbamoyltransferase TobZ A
ec: 2.1.3.-
mutation: M473I
Source :
mol_id organism_scientific organism_common expression_system
1 Streptoalloteichus tenebrarius  (taxid:1933) Streptomyces tenebrarius Streptomyces lividans  (taxid:457428)
strain: DSM40770T
gene: tacA, tobZ
expression_system_strain: TK24
expression_system_vector_type: plasmid
expression_system_plasmid: pUWL201PW
Authors : Parthier, C., Stubbs, M.T., Goerlich, S., Jaenecke, F.
Keywords : antibiotic biosynthesis, substrate assisted catalysis, substrate channeling, adenylation, structural enzymology, enzyme evolution, TRANSFERASE
Exp. method : X-RAY DIFFRACTION ( 2.900 Å )
Citation :

The O-Carbamoyltransferase TobZ Catalyzes an Ancient Enzymatic Reaction.

Parthier, C.,Gorlich, S.,Jaenecke, F.  et al.
(2012)  Angew.Chem.Int.Ed.Engl.  51 : 4046 - 4052

PubMed: 22383337
DOI: 10.1002/anie.201108896

Reaction

Chain : A
UniProt : Q70IY1 (TOBZ_STRSD)
Reaction : Tobramycin + carbamoyl phosphate + ATP + H(2)O = nebramycin 5' + AMP + diphosphate + phosphate.
Kanamycin A + carbamoyl phosphate + ATP + H(2)O = 6''-O-carbamoylkanamycin A + AMP + diphosphate + phosphate.