Brand  (β version)

PDB ID: 3VER

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Ligands
Code Name Style Show Link
CA0 5'-O-[(S)-(carbamoyloxy)(hydroxy)phosphoryl]adenosine PoSSuM
FE2 Fe (II) ion PoSSuM
NA Sodium ion PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 3VER   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE
Title : Crystal structure of the O-carbamoyltransferase TobZ in complex with carbamoyl adenylate intermediate
Release Data : 2012-01-25
Compound :
mol_id molecule chains
1 O-carbamoyltransferase TobZ A
ec: 2.1.3.-
Source :
mol_id organism_scientific organism_common expression_system
1 Streptoalloteichus tenebrarius  (taxid:1933) Streptomyces tenebrarius Streptomyces lividans  (taxid:457428)
strain: DSM40770T
gene: tacA, tobZ
expression_system_strain: TK24
expression_system_vector_type: plasmid
expression_system_plasmid: pUWL201PW
Authors : Parthier, C., Stubbs, M.T., Goerlich, S., Jaenecke, F.
Keywords : antibiotic biosynthesis, substrate assisted catalysis, substrate channeling, adenylation, structural enzymology, enzyme evolution, TRANSFERASE
Exp. method : X-RAY DIFFRACTION ( 2.340 Å )
Citation :

The O-Carbamoyltransferase TobZ Catalyzes an Ancient Enzymatic Reaction.

Parthier, C.,Gorlich, S.,Jaenecke, F.  et al.
(2012)  Angew.Chem.Int.Ed.Engl.  51 : 4046 - 4052

PubMed: 22383337
DOI: 10.1002/anie.201108896

Reaction

Chain : A
UniProt : Q70IY1 (TOBZ_STRSD)
Reaction: EC: Evidence:
Physiological Direction:
ATP + carbamoyl phosphate + H2O + tobramycin = AMP + diphosphate + H(+) + nebramycin 5' + phosphate 6.1.2.2 PubMed:22383337
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ATP + carbamoyl phosphate + H2O + kanamycin A = 6''-O- carbamoylkanamycin A + AMP + diphosphate + H(+) + phosphate 6.1.2.2 PubMed:22383337
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ATP + carbamoyl phosphate + H2O = carbamoyl adenylate + diphosphate + phosphate - PubMed:22383337
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carbamoyl adenylate + tobramycin = AMP + H(+) + nebramycin 5' - PubMed:22383337
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carbamoyl adenylate + kanamycin A = 6''-O-carbamoylkanamycin A + AMP + H(+) - PubMed:22383337
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