PDB ID: 3TOF
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|Header :||HYDROLASE/HYDROLASE INHIBITOR|
|Title :||HIV-1 Protease - Epoxydic Inhibitor Complex (pH 6 - Orthorombic Crystal form P212121)|
|Release Data :||2012-08-15|
|Authors :||Geremia, S., Olajuyigbe, F.M., Ajele, J.O., Demitri, N., Randaccio, L., Wuerges, J., Benedetti, L., Campaner, P., Berti, F.|
|Keywords :||HIV PR, epoxide, in-crystal reaction, hydrolase, HYDROLASE-HYDROLASE INHIBITOR complex|
|Exp. method :||X-RAY DIFFRACTION ( 1.45 Å )|
|Chain :||A, B|
|UniProt :||P03367 (POL_HV1BR)|
|Reaction :||Specific for a P1 residue that is hydrophobic, and P1' variable, but often Pro.
Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.
3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid.
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).