Brand  (β version)

PDB ID: 3TAG

Hetero Atom Contents

  The number of atoms exceeds 100,000.
  So, it can not be displayed here.

Select unit:

Select hetatm:   

close
information
centroid:
interaction residue:

Select chain:   Sequence  

Data format:   

Color scheme of protein:

Ligands
Code Name Style Show Link
SO4 Sulfate ion PoSSuM
Non-standard Residues
Code Name Show
C37 5-fluoro-2'-deoxy-cytidine-5'-monophosphate
Glycosylation
Code Name Emphasize
Modification
Code Name Show

Structure summary

Code : 3TAG   PDBj   RCSB PDB   PDBe
Header : Transferase/DNA
Title : 5-fluorocytosine paired with dAMP in RB69 gp43
Release Data : 2011-11-09
Compound :
mol_id molecule chains synonym
1 DNA-directed DNA polymerase A,B,C,D Gp43
ec: 2.7.7.7,3.1.11.-
mutation: D222A, D327A
mol_id molecule chains
2 DNA (5'-D(*CP*CP*(C37)P*GP*GP*TP*AP*TP*GP*AP*CP*AP*GP*CP*CP*GP*CP*G)-3') E,I,G,K
other_details: Template DNA
mol_id molecule chains
3 DNA (5'-D(*GP*CP*GP*GP*CP*TP*GP*TP*CP*AP*TP*AP*CP*CP*A)-3') F,J,H,L
other_details: Primer DNA
Source :
mol_id organism_scientific expression_system
1 Escherichia phage RB69  (taxid:12353) Escherichia coli  (taxid:562)
gene: 43
mol_id organism_scientific
2 Synthetic construct  (taxid:32630)
synthetic: yes
mol_id organism_scientific
3 Synthetic construct  (taxid:32630)
synthetic: yes
Authors : Zahn, K.E.
Keywords : Transferase-DNA complex
Exp. method : X-RAY DIFFRACTION ( 2.95 Å )
Citation :

The miscoding potential of 5-hydroxycytosine arises due to template instability in the replicative polymerase active site.

Zahn, K.E.,Averill, A.,Wallace, S.S.  et al.
(2011)  Biochemistry  50 : 10350 - 10358

PubMed: 22026756
DOI: 10.1021/bi201219s

Reaction

Chain : A, B, C, D
UniProt : Q38087 (DPOL_BPR69)
Reaction: EC: Evidence:
Physiological Direction:
a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1) 2.7.7.7 HAMAP- Rule:MF_04100, PubMed:17098747, PubMed:18503083, PubMed:20795733, PubMed:22616982, PubMed:24116139
-