Brand  (β version)

PDB ID: 3S44

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FN5 Cmp-3f(a)-neu5ac PoSSuM

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Glycosylation
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Structure summary

Code : 3S44   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE
Title : Crystal Structure of Pasteurella multocida sialyltransferase M144D mutant with CMP bound
Release Data : 2012-08-15
Compound :
mol_id molecule chains
1 Alpha-2,3/2,6-sialyltransferase/sialidase A
ec: 2.4.99.-
fragment: UNP residues 26-412
mutation: M144D
Source :
mol_id organism_scientific expression_system
1 Pasteurella multocida  (taxid:747) Escherichia coli  (taxid:469008)
strain: P-1059
gene: homologue of Pm0188
expression_system_strain: BL21(DE3)
expression_system_vector_type: plasmid
expression_system_plasmid: pET23a(+)
Authors : Sugiarto, G., Lau, K., Li, Y., Lim, S., Ames, J.B., Le, D.-T., Fisher, A.J., Chen, X.
Keywords : GT-B fold, Sialyltransferase, TRANSFERASE
Exp. method : X-RAY DIFFRACTION ( 1.45 Å )
Citation :

A Sialyltransferase Mutant with Decreased Donor Hydrolysis and Reduced Sialidase Activities for Directly Sialylating Lewis(x).

Sugiarto, G.,Lau, K.,Qu, J.  et al.
(2012)  Acs Chem.Biol.  7 : 1232 - 1240

PubMed: 22583967
DOI: 10.1021/cb300125k

Reaction

Chain : A
UniProt : Q15KI8 (PMST1_PASMD)
Reaction: EC: Evidence:
Physiological Direction:
a beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl derivative + CMP-N-acetyl-beta-neuraminate = an N-acetyl-alpha- neuraminyl-(2->3)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D- glucosaminyl derivative + CMP + H(+) 2.4.3.6 PubMed:16351087, PubMed:35872821
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an N(4)-{beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->2)-alpha-D-Man- (1->3)-[beta-D-GlcNAc-(1->4)]-[beta-D-Gal-(1->4)-beta-D-GlcNAc- (1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)- beta-D-GlcNAc}-L-asparaginyl-[protein] + 2 CMP-N-acetyl-beta- neuraminate = an N(4)-{alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->4)-beta-D- GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->4)]-[alpha- Neu5Ac-(2->3)-beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->2)-alpha-D-Man- (1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc}-L- asparaginyl-[protein] + 2 CMP + 2 H(+) - PubMed:35872821
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an N(4)-{beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->2)-alpha-D-Man- (1->3)-[beta-D-GlcNAc-(1->4)]-[beta-D-GlcNAc-(1->2)-alpha-D-Man- (1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc}-L- asparaginyl-[protein] + CMP-N-acetyl-beta-neuraminate = an N(4)-{alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->2)- alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->4)]-[beta-D-GlcNAc-(1->2)- alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D- GlcNAc}-L-asparaginyl-[protein] + CMP + H(+) - PubMed:35872821
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an N(4)-{beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D- GlcNAc-(1->4)]-[beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->2)-alpha-D-Man- (1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc}-L- asparaginyl-[protein] + CMP-N-acetyl-beta-neuraminate = an N(4)-{beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->4)]- [alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->2)-alpha-D- Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc}-L- asparaginyl-[protein] + CMP + H(+) - PubMed:35872821
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an N(4)-{alpha-Neu5Ac-(2->6)-beta-D-Gal-(1->4)-beta-D-GlcNAc- (1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->4)]-[beta-D-Gal-(1->4)- beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D- GlcNAc-(1->4)-beta-D-GlcNAc}-L-asparaginyl-[protein] + CMP-N-acetyl- beta-neuraminate = an N(4)-{alpha-Neu5Ac-(2->6)-beta-D-Gal-(1->4)- beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->4)]- [alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->2)-alpha-D- Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc}-L- asparaginyl-[protein] + CMP + H(+) - PubMed:35872821
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an N(4)-{beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->2)-alpha-D-Man- (1->3)-[beta-D-GlcNAc-(1->4)]-[alpha-Neu5Ac-(2->6)-beta-D-Gal-(1->4)- beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D- GlcNAc-(1->4)-beta-D-GlcNAc}-L-asparaginyl-[protein] + CMP-N-acetyl- beta-neuraminate = an N(4)-{alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->4)- beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->4)]- [alpha-Neu5Ac-(2->6)-beta-D-Gal-(1->4)-beta-D-GlcNAc-(1->2)-alpha-D- Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc}-L- asparaginyl-[protein] + CMP + H(+) - PubMed:35872821
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