Brand  (β version)

PDB ID: 3OTU

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Ligands
Code Name Style Show Link
BI4 3-(1h-indol-3-yl)-4-{1-[2-(1-methylpyrrolidin-2-yl)ethyl]-1h-indol-3-yl}-1h-pyrrole-2,5-dione PoSSuM
CL Chloride ion PoSSuM
J30 4-[4-(naphthalen-1-ylmethyl)piperazin-1-yl]-4-oxobutane-1-thiol PoSSuM
SO4 Sulfate ion PoSSuM
Non-standard Residues
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SEP Phosphoserine
Glycosylation
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Structure summary

Code : 3OTU   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE/TRANSFERASE ACTIVATOR
Title : PDK1 mutant bound to allosteric disulfide fragment activator JS30
Release Data : 2011-03-23
Compound :
mol_id molecule chains synonym
1 3-phosphoinositide-dependent protein kinase 1 A hPDK1
ec: 2.7.11.1
fragment: Catalytic domain (UNP residues 51-359)
mutation: T148C
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Spodoptera frugiperda  (taxid:7108)
gene: PDK1, PDPK1
expression_system_strain: SF21
expression_system_vector_type: Baculovirus
Authors : Sadowsky, J.D., Wells, J.A.
Keywords : PIF pocket, activation loop, C helix, Ser/Thr-kinase, AGC kinase, allostery, transferase, allosteric activator, bisindolylmaleimide, phosphorylation, disulfide, kinase, PDK1, TRANSFERASE-TRANSFERASE ACTIVATOR complex
Exp. method : X-RAY DIFFRACTION ( 2.1013 Å )
Citation :

Turning a protein kinase on or off from a single allosteric site via disulfide trapping.

Sadowsky, J.D.,Burlingame, M.A.,Wolan, D.W.  et al.
(2011)  Proc.Natl.Acad.Sci.USA  108 : 6056 - 6061

PubMed: 21430264
DOI: 10.1073/pnas.1102376108

Reaction

Chain : A
UniProt : O15530 (PDPK1_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+) 2.7.11.1 PubMed:9368760
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L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+) 2.7.11.1 PubMed:9368760
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