Brand  (β version)

PDB ID: 3NN6

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Ligands
Code Name Style Show Link
FB0 (2r,3s,4s)-5-({[(acetylcarbamoyl)amino]methyl}[(3s,4r)-6-amino-3,4-dimethylhexyl]amino)-2,3,4-trihydroxypentyl [(2r,3s,4r,5r)-5-(6-amino-9h-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl dihydrogen diphosphate (non-preferred name) PoSSuM
GP7 (1r)-2-{[(S)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-1-[(pentadecanoyloxy)methyl]ethyl (12e)-hexadeca-9,12-dienoate PoSSuM
HNK 5-[(2r)-1-methylpyrrolidin-2-yl]pyridin-2-ol PoSSuM
Non-standard Residues
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Structure summary

Code : 3NN6   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE/OXIDOREDUCTASE INHIBITOR
Title : Crystal structure of inhibitor-bound in active centre 6-hydroxy-L-nicotine oxidase from Arthrobacter nicotinovorans
Release Data : 2011-03-23
Compound :
mol_id molecule chains
1 6-hydroxy-L-nicotine oxidase X
ec: 1.5.3.5
Source :
mol_id organism_scientific expression_system
1 Arthrobacter nicotinovorans  (taxid:29320) Escherichia coli  (taxid:562)
gene: 6-HLNO
expression_system_strain: JM105
expression_system_vector_type: plasmid
expression_system_plasmid: pTrc99A
Authors : Kachalova, G.S., Bartunik, H.D.
Keywords : ENANTIOMERIC SUBSTRATE- INHIBITOR, FLAVOENZYMES, NICOTINE DEGRADATION, FAD-binding fold, amino oxidase, FAD binding, cytosol, OXIDOREDUCTASE-OXIDOREDUCTASE INHIBITOR complex
Exp. method : X-RAY DIFFRACTION ( 2.19 Å )
Citation :

Crystal structure analysis of free and substrate-bound 6-hydroxy-L-nicotine oxidase from Arthrobacter nicotinovorans.

Kachalova, G.S.,Bourenkov, G.P.,Mengesdorf, T.  et al.
(2010)  J.Mol.Biol.  396 : 785 - 799

PubMed: 20006620
DOI: 10.1016/j.jmb.2009.12.009

Crystal structure analysis of free and substrate-bound 6-hydroxy-L-nicotine from Arthrobacter nicotinovorans

Kachalova, G.S.,Bourenkov, G.P.,Mengesdorf, T.  et al.
(2010)  J.Mol.Biol.  396 : 785 - 799

Reaction

Chain : X
UniProt : Q93NH4 (HLNO_PAENI)
Reaction: EC: Evidence:
Physiological Direction:
(S)-6-hydroxynicotine + H2O + O2 = 6-hydroxypseudooxynicotine + H2O2 1.5.3.5 PubMed:21383134, PubMed:26744768, PubMed:28080034, PubMed:4965794, PubMed:5646150, PubMed:5849820
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(S)-6-hydroxynicotine + O2 = 6-hydroxy-N-methylmyosmine + H2O2 - PubMed:21383134, PubMed:26744768, PubMed:28080034, PubMed:4965794
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