Brand  (β version)

PDB ID: 3NGY

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Ligands
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CO Cobalt (II) ion PoSSuM
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Glycosylation
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Structure summary

Code : 3NGY   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : Crystal structure of RNase T (E92G mutant)
Release Data : 2011-02-16
Compound :
mol_id molecule chains synonym
1 Ribonuclease T A,B,C,D RNase T, Exoribonuclease T
ec: 3.1.13.-
mutation: E92G
mol_id molecule chains
2 his tag sequence E
Source :
mol_id organism_scientific expression_system
1 Escherichia coli  (taxid:562) Escherichia coli  (taxid:562)
strain: JM109 / ATCC 53323
gene: rnt
expression_system_strain: RIPL
expression_system_vector_type: plasmid
expression_system_plasmid: pET28a
mol_id organism_scientific expression_system
2 Escherichia coli  (taxid:562) Escherichia coli  (taxid:562)
strain: JM109 / ATCC 53323
gene: rnt
expression_system_strain: RIPL
expression_system_vector_type: plasmid
expression_system_plasmid: pET28a
Authors : Hsiao, Y.-Y., Yuan, H.S.
Keywords : exoribonuclease, RNA processing, RNA maturation, protein-DNA interactions, exo-nuclease, HYDROLASE
Exp. method : X-RAY DIFFRACTION ( 2.2040 Å )
Citation :

Structural basis for RNA trimming by RNase T in stable RNA 3'-end maturation

Hsiao, Y.-Y.,Yang, C.-C.,Lin, C.L.  et al.
(2011)  Nat.Chem.Biol.  7 : 236 - 243

PubMed: 21317904
DOI: 10.1038/nchembio.524

Reaction

Chain : A, B, C, D
UniProt : P30014 (RNT_ECOLI)
Reaction : -
Cofactor: Evidence: Note:
Mg(2+) ECO:0000255 | HAMAP-Rule:MF_00157
ECO:0000269 | PubMed:12364334
ECO:0000269 | PubMed:17437714
ECO:0000269 | PubMed:21317904
ECO:0000269 | PubMed:22718982
Binds two Mg(2+) per subunit. The active form of the enzyme binds two Mg(2+) ions in its active site. The first Mg(2+) forms only one salt bridge with the protein.