Het-PDB Navi2 (PDB: 3K5L)

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Ligands
Code	Name		Style	Show	Link
DTP	2'-deoxyadenosine 5'-triphosphate				PoSSuM
MG	Magnesium ion				PoSSuM

Non-standard Residues
Code	Name	Show
3DR	1',2'-dideoxyribofuranose-5'-phosphate
DOC	2',3'-dideoxycytidine-5'-monophosphate

Glycosylation
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Modification
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Download interaction data: 3K5L

Code :

3K5L PDBj RCSB PDB PDBe

Header :

TRANSFERASE/DNA

Title :

Crystal structure of E.coli Pol II-abasic DNA-dATP Lt(0, 3) ternary complex

Release Data :

2010-02-02

Compound :

mol_id	molecule	chains	synonym
1	DNA polymerase II	A	Pol II
	ec: 2.7.7.7 mutation: D335N

mol_id	molecule	chains
2	DNA (5'-D(TPAPTP(3DR)PGPTPAPCPGPCPTPAPGPGPCPAPCPA)-3')	T
	other_details: Abasic template

mol_id	molecule	chains
3	DNA (5'-D(GPTPGPCPCPTPAPGPCPGPTPAP*(DOC))-3')	P
	other_details: Primer

Source :

mol_id	organism_scientific	expression_system
1	Escherichia coli (taxid:562)	Escherichia coli (taxid:562)
	strain: K-12 gene: b0060, dinA, JW0059, polB expression_system_strain: BL21(DE3) expression_system_vector_type: PLASMID expression_system_plasmid: Modified PET28

mol_id	organism_scientific
2
	synthetic: yes

mol_id	organism_scientific
3
	synthetic: yes

Authors :

Yang, W., Wang, F.

Keywords :

DNA damage, DNA repair, DNA-binding, DNA-directed DNA polymerase, Nucleotidyltransferase, SOS response, Transferase, TRANSFERASE-DNA complex

Exp. method :

X-RAY DIFFRACTION ( 2.700 Å )

Citation :

Structural insight into translesion synthesis by DNA Pol II.

Wang, F.,Yang, W.
(2009) Cell(Cambridge,Mass.) 139 : 1279 - 1289

Chain :

UniProt :

P21189 (DPO2_ECOLI)

Reaction:	EC:	Evidence:
Reaction:	Physiological Direction:	Evidence:
a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)	2.7.7.7	-
	-	-

PDB ID: 3K5L