Brand  (β version)

PDB ID: 3HTD

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Ligands
Code Name Style Show Link
BME Beta-mercaptoethanol PoSSuM
JZ5 (nz)-N-(thiophen-2-ylmethylidene)hydroxylamine PoSSuM
PO4 Phosphate ion PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 3HTD   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : (Z)-Thiophene-2-carboxaldoxime in complex with T4 lysozyme L99A/M102Q
Release Data : 2009-11-03
Compound :
mol_id molecule chains synonym
1 Lysozyme A Lysis protein, Muramidase, Endolysin
ec: 3.2.1.17
mutation: S38D,L99A,M102Q,N144D
Source :
mol_id organism_scientific organism_common expression_system
1 Enterobacteria phage T4  (taxid:10665) Bacteriophage T4 Escherichia coli  (taxid:562)
strain: Enterobacteria Phage T4 Sensu Lato
gene: E
expression_system_vector_type: plasmid
expression_system_plasmid: M13
Authors : Boyce, S.E., Mobley, D.L., Rocklin, G.J., Graves, A.P., Dill, K.A., Shoichet, B.K.
Keywords : HYDROLASE, GLYCOSIDASE, BACTERIOLYTIC ENZYME, Antimicrobial
Exp. method : X-RAY DIFFRACTION ( 1.400 Å )
Citation :

Predicting ligand binding affinity with alchemical free energy methods in a polar model binding site.

Boyce, S.E.,Mobley, D.L.,Rocklin, G.J.  et al.
(2009)  J.Mol.Biol.  394 : 747 - 763

PubMed: 19782087
DOI: 10.1016/j.jmb.2009.09.049

Reaction

Chain : A
UniProt : P00720 (ENLYS_BPT4)
Reaction: EC: Evidence:
Physiological Direction:
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins. 3.2.1.17 HAMAP-Rule:MF_04110, PubMed:4865643
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