Brand  (β version)

PDB ID: 3GUK

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Ligands
Code Name Style Show Link
MBN Toluene PoSSuM
CA Calcium ion PoSSuM
CL Chloride ion PoSSuM
Non-standard Residues
Code Name Show
CME S,S-(2-hydroxyethyl)thiocysteine
Glycosylation
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Modification
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Structure summary

Code : 3GUK   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : T4 lysozyme M102E/L99A mutant with buried charge in apolar cavity--Toluene binding
Release Data : 2009-08-25
Compound :
mol_id molecule chains synonym
1 Lysozyme A,B Lysis protein, Muramidase, Endolysin
ec: 3.2.1.17
mutation: T21C/S38D/L99A/M102E/E108V/S117V/T142C/N144D
Source :
mol_id organism_scientific expression_system
1 Enterobacteria phage T4  (taxid:10665) Escherichia coli  (taxid:562)
gene: E
expression_system_strain: RR1
expression_system_vector_type: plasmid
expression_system_plasmid: p1403
Authors : Liu, L., Matthews, B.W.
Keywords : T4 lysozyme, apolar cavity, buried charge, ligand binding, Antimicrobial, Bacteriolytic enzyme, Glycosidase, Hydrolase
Exp. method : X-RAY DIFFRACTION ( 1.85 Å )
Citation :

Use of stabilizing mutations to engineer a charged group within a ligand-binding hydrophobic cavity in T4 lysozyme.

Liu, L.,Baase, W.A.,Michael, M.M.  et al.
(2009)  Biochemistry  48 : 8842 - 8851

PubMed: 19663503
DOI: 10.1021/bi900685j

Reaction

Chain : A, B
UniProt : P00720 (ENLYS_BPT4)
Reaction: EC: Evidence:
Physiological Direction:
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins. 3.2.1.17 HAMAP-Rule:MF_04110, PubMed:4865643
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