Brand  (β version)

PDB ID: 3CV6

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Ligands
Code Name Style Show Link
NAP Nadp nicotinamide-adenine-dinucleotide phosphate PoSSuM
HXS 4-[(1r,2s)-1-ethyl-2-(4-hydroxyphenyl)butyl]phenol PoSSuM
BME Beta-mercaptoethanol PoSSuM
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Structure summary

Code : 3CV6   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : The crystal structure of mouse 17-alpha hydroxysteroid dehydrogenase GG225.226PP mutant in complex with inhibitor and cofactor NADP+.
Release Data : 2009-03-03
Compound :
mol_id molecule chains synonym
1 Aldo-keto reductase family 1 member C21 A,B 17-alpha-hydroxysteroid dehydrogenase, 17-alpha-HSD, 3-alpha-hydroxysteroid dehydrogenase, Dihydrodiol dehydrogenase type 1, DD1, Dihydrodiol dehydrogenase type 3, DD3
ec: 1.1.1.209
mutation: G225P, G226P
Source :
mol_id organism_scientific organism_common expression_system
1 Mus musculus  (taxid:10090) Mouse Escherichia coli  (taxid:562)
gene: Akr1c21
expression_system_strain: JM109
expression_system_vector_type: Plasmid
expression_system_plasmid: pkk223-3
Authors : Dhagat, U., El-Kabbani, O.
Keywords : Aldo-keto reductase, Hydroxysteroid Dehydrogenase, Ternary Complex, Hexestrol, Lipid metabolism, NADP, Oxidoreductase, Phosphoprotein, Steroid metabolism
Exp. method : X-RAY DIFFRACTION ( 2.10 Å )
Citation :

Structure of the G225P/G226P mutant of mouse 3(17)alpha-hydroxysteroid dehydrogenase (AKR1C21) ternary complex: implications for the binding of inhibitor and substrate.

Dhagat, U.,Endo, S.,Mamiya, H.  et al.
(2009)  Acta Crystallogr.,Sect.D  65 : 257 - 265

PubMed: 19237748
DOI: 10.1107/S0907444908044028

Reaction

Chain : A, B
UniProt : Q91WR5 (AK1CL_MOUSE)
Reaction: EC: Evidence:
Physiological Direction:
androsterone + NADP(+) = 5alpha-androstan-3,17-dione + NADPH + H(+) 1.1.1.209 PubMed:15577209, PubMed:17442338, PubMed:20124700
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androsterone + NAD(+) = 5alpha-androstan-3,17-dione + NADH + H(+) 1.1.1.209 PubMed:15577209, PubMed:17442338, PubMed:20124700
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