Brand  (β version)

PDB ID: 3V8D

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Ligands
Code Name Style Show Link
0GV (3beta,8alpha,9beta)-3-hydroxycholest-5-en-7-one PoSSuM
HEM Protoporphyrin ix containing Fe PoSSuM
SO4 Sulfate ion PoSSuM
UNX Unknown atom or ion PoSSuM
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Structure summary

Code : 3V8D   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : Crystal structure of human CYP7A1 in complex with 7-ketocholesterol
Release Data : 2013-01-09
Compound :
mol_id molecule chains synonym
1 Cholesterol 7-alpha-monooxygenase A,B CYPVII, Cholesterol 7-alpha-hydroxylase, Cytochrome P450 7A1
ec: 1.14.13.17
fragment: UNP residues 25-503
mutation: T104L
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Escherichia coli  (taxid:562)
gene: CYP7A1, CYP7
expression_system_strain: JM109
expression_system_vector_type: plasmid
expression_system_plasmid: pCW
Authors : Strushkevich, N., Tempel, W., MacKenzie, F., Wernimont, A.K., Arrowsmith, C.H., Edwards, A.M., Bountra, C., Weigelt, J., Usanov, S.A., Park, H., Structural Genomics Consortium (SGC)
Keywords : cytochrome, OXIDOREDUCTASE, Structural Genomics, Structural Genomics Consortium, SGC
Exp. method : X-RAY DIFFRACTION ( 1.9000 Å )
Citation :

Crystal structure of human CYP7A1 in complex with 7-ketocholesterol

Strushkevich, N.,Tempel, W.,MacKenzie, F.  et al.
To be Published 

Reaction

Chain : A, B
UniProt : P22680 (CP7A1_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
cholesterol + O2 + reduced [NADPH--hemoprotein reductase] = 7alpha-hydroxycholesterol + H(+) + H2O + oxidized [NADPH--hemoprotein reductase] 1.14.14.23 PubMed:11013305, PubMed:12077124, PubMed:2384150
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4beta-hydroxycholesterol + O2 + reduced [NADPH--hemoprotein reductase] = 4beta,7alpha-dihydroxycholesterol + H(+) + H2O + oxidized [NADPH--hemoprotein reductase] - PubMed:12077124
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lathosterol + O2 + reduced [NADPH--hemoprotein reductase] = 7alpha,8alpha-epoxy-5alpha-cholestan-3beta-ol + H(+) + H2O + oxidized [NADPH--hemoprotein reductase] - PubMed:21813643
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lathosterol + O2 + reduced [NADPH--hemoprotein reductase] = 5alpha-cholestan-7-oxo-3beta-ol + H(+) + H2O + oxidized [NADPH-- hemoprotein reductase] - PubMed:21813643
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7-dehydrocholesterol + O2 + reduced [NADPH--hemoprotein reductase] = 7-oxocholesterol + H(+) + H2O + oxidized [NADPH-- hemoprotein reductase] - PubMed:21813643
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(24S)-hydroxycholesterol + O2 + reduced [NADPH--hemoprotein reductase] = (24S)-7alpha-dihydroxycholesterol + H(+) + H2O + oxidized [NADPH--hemoprotein reductase] 1.14.14.26 PubMed:11013305
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(24R)-hydroxycholesterol + O2 + reduced [NADPH--hemoprotein reductase] = (24R)-7alpha-dihydroxycholesterol + H(+) + H2O + oxidized [NADPH--hemoprotein reductase] - PubMed:11013305
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