Brand  (β version)

PDB ID: 3V3B

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Ligands
Code Name Style Show Link
CL Chloride ion PoSSuM
Non-standard Residues
Code Name Show
0EH (2r)-2-amino-2-methylnonanoic acid
MK8 2-methyl-L-norleucine
Glycosylation
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Structure summary

Code : 3V3B   PDBj   RCSB PDB   PDBe
Header : ligase/ligase inhibitor
Title : Structure of the Stapled p53 Peptide Bound to Mdm2
Release Data : 2012-01-18
Compound :
mol_id molecule chains synonym
1 E3 ubiquitin-protein ligase Mdm2 A,B Double minute 2 protein, Hdm2, Oncoprotein Mdm2, p53-binding protein Mdm2
ec: 6.3.2.-
mol_id molecule chains
2 SAH-p53-8 stapled-peptide C,D
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Escherichia coli  (taxid:562)
gene: MDM2
expression_system_strain: BL21(DE3)
expression_system_plasmid: pET-20
mol_id organism_scientific
2
synthetic: yes
other_details: synthetic cyclic peptide
Authors : Baek, S., Kutchukian, P.S., Verdine, G.L., Huber, R., Holak, T.A., Ki Won, L., Popowicz, G.M.
Keywords : oncoprotein, cell cycle, DNA repair, cancer, p53-derived peptide, aliphatic staple, ligase-ligase inhibitor complex
Exp. method : X-RAY DIFFRACTION ( 2.00 Å )
Citation :

Structure of the stapled p53 peptide bound to Mdm2.

Baek, S.,Kutchukian, P.S.,Verdine, G.L.  et al.
(2012)  J.Am.Chem.Soc.  134 : 103 - 106

PubMed: 22148351
DOI: 10.1021/ja2090367

Reaction

Chain : A, B
UniProt : Q00987 (MDM2_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L- cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine. 2.3.2.27 PubMed:12821780
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