Brand  (β version)

PDB ID: 3UWL

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Ligands
Code Name Style Show Link
SO4 Sulfate ion PoSSuM
FFO N-[4-({[(6s)-2-amino-5-formyl-4-oxo-3,4,5,6,7,8-hexahydropteridin-6-yl]methyl}amino)benzoyl]-L-glutamic acid PoSSuM
EDO 1,2-ethanediol PoSSuM
Non-standard Residues
Code Name Show
CME S,S-(2-hydroxyethyl)thiocysteine
Glycosylation
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Modification
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Structure summary

Code : 3UWL   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE
Title : Crystal structure of Enteroccocus faecalis thymidylate synthase (EfTS) in complex with 5-formyl tetrahydrofolate
Release Data : 2012-08-29
Compound :
mol_id molecule chains synonym
1 Thymidylate synthase A,C TS, TSase
ec: 2.1.1.45
mol_id molecule chains synonym
2 Thymidylate synthase B,D TS, TSase
ec: 2.1.1.45
Source :
mol_id organism_scientific expression_system
1 Enterococcus faecalis  (taxid:1351) Escherichia coli  (taxid:469008)
gene: EF_1576, thyA
expression_system_strain: BL21(DE3)
expression_system_vector_type: plasmid
expression_system_plasmid: pET30a
mol_id organism_scientific expression_system
2 Enterococcus faecalis  (taxid:1351) Escherichia coli  (taxid:469008)
gene: EF_1576, thyA
expression_system_strain: BL21(DE3)
expression_system_vector_type: plasmid
expression_system_plasmid: pET30a
Authors : Pozzi, C., Catalano, A., Cortesi, D., Luciani, R., Ferrari, S., Fritz, T., Costi, M.P., Mangani, S.
Keywords : methyltransferase, TRANSFERASE
Exp. method : X-RAY DIFFRACTION ( 2.07 Å )
Citation :

The structure of Enterococcus faecalis thymidylate synthase provides clues about folate bacterial metabolism.

Pozzi, C.,Ferrari, S.,Cortesi, D.  et al.
(2012)  Acta Crystallogr.,Sect.D  68 : 1232 - 1241

PubMed: 22948925
DOI: 10.1107/S0907444912026236

Reaction

Chain : B, D
UniProt : Q834R3 (TYSY_ENTFA)
Reaction: EC: Evidence:
Physiological Direction:
(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP = 7,8- dihydrofolate + dTMP 2.1.1.45 HAMAP-Rule:MF_00008
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