Brand  (β version)

PDB ID: 3UTD

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Ligands
Code Name Style Show Link
F3S Fe3-s4 cluster PoSSuM
0CJ 4-oxopentyl trihydrogen diphosphate PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 3UTD   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : Ec_IspH in complex with 4-oxopentyl diphosphate
Release Data : 2012-09-05
Compound :
mol_id molecule chains
1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase A,B
ec: 1.17.1.2
Source :
mol_id organism_scientific expression_system
1 Escherichia coli  (taxid:83333) Escherichia coli  (taxid:562)
strain: K12
gene: ispH, lytB, yaaE, b0029, JW0027
expression_system_strain: XL1 blue
expression_system_vector_type: Plasmid
expression_system_plasmid: pQE30
Authors : Span, I., Wang, K., Wang, W., Zhang, Y., Bacher, A., Eisenreich, W., Schulz, C., Oldfield, E., Groll, M.
Keywords : iron-sulfur protein, IspH, LytB, isoprenoid biosynthesis, non-mevalonate pathway, OXIDOREDUCTASE
Exp. method : X-RAY DIFFRACTION ( 1.70 Å )
Citation :

Discovery of acetylene hydratase activity of the iron-sulphur protein IspH.

Span, I.,Wang, K.,Wang, W.  et al.
(2012)  Nat Commun  3 : 1042 - 1042

PubMed: 22948824
DOI: 10.1038/ncomms2052

Reaction

Chain : A, B
UniProt : P62623 (ISPH_ECOLI)
Reaction: EC: Evidence:
Physiological Direction:
isopentenyl diphosphate + 2 oxidized [2Fe-2S]-[ferredoxin] + H2O = (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 reduced [2Fe- 2S]-[ferredoxin] + 2 H(+) 1.17.7.4 PubMed:12198182, PubMed:12571359, PubMed:12706830
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dimethylallyl diphosphate + 2 oxidized [2Fe-2S]-[ferredoxin] + H2O = (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 reduced [2Fe- 2S]-[ferredoxin] + 2 H(+) 1.17.7.4 PubMed:12198182, PubMed:12571359, PubMed:12706830
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Cofactor: Evidence: Note:
[4Fe-4S] cluster ECO:0000269 | PubMed:12706830
ECO:0000269 | PubMed:20080550
ECO:0000269 | PubMed:22012762
ECO:0000269 | PubMed:22137895
ECO:0000269 | PubMed:22687151
Was shown to bind 1 [3Fe-4S] cluster per subunit (PubMed:15469281, PubMed:19569147, PubMed:20080550). However, it initially contains a [4Fe-4S] cluster which easily degrades into a [3Fe-4S] form in the presence of oxygen (PubMed:12706830, PubMed:20080550, PubMed:22137895, PubMed:22687151).