Brand  (β version)

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information
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interaction residue:

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Ligands
Code Name Link
0CG Prop-2-yn-1-yl trihydrogen diphosphate
SF4 Iron/sulfur cluster
Code : 3URK
Header : OXIDOREDUCTASE
Title : IspH in complex with propynyl diphosphate (1061)
Release Data : 2012-09-05
Compound :
mol_id molecule chains
1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase A,B
ec: 1.17.1.2
Source :
mol_id organism_scientific expression_system
1 Escherichia coli  (taxid:83333) Escherichia coli  (taxid:562)
strain: K12
gene: ispH, lytB, yaaE, b0029, JW0027
expression_system_strain: XL1 blue
expression_system_vector_type: Plasmid
expression_system_plasmid: pQE30
Authors : Span, I., Wang, K., Wang, W., Zhang, Y., Bacher, A., Eisenreich, W., Schulz, C., Oldfield, E., Groll, M.
Keywords : iron-sulfur protein, IspH, LytB, isoprenoid biosynthesis, non-mevalonate pathway, OXIDOREDUCTASE
Exp. method : X-RAY DIFFRACTION ( 1.50 Å )
Citation :

Discovery of acetylene hydratase activity of the iron-sulphur protein IspH.

Span, I.,Wang, K.,Wang, W.  et al.
(2012)  Nat Commun  3 : 1042 - 1042

PubMed: 22948824
DOI: 10.1038/ncomms2052

Chain : A, B
UniProt : P62623 (ISPH_ECOLI)
Reaction : Isopentenyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H(2)O = (E)-4-hydroxy-3- methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron- sulfur] cluster + 2 H(+).
Dimethylallyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H(2)O = (E)-4-hydroxy-3- methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron- sulfur] cluster + 2 H(+).