Brand  (β version)

PDB ID: 3U2Z

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Ligands
Code Name Style Show Link
07T 6-(3-aminobenzyl)-4-methyl-2-methylsulfinyl-4,6-dihydro-5h-thieno[2',3':4,5]pyrrolo[2,3-D]pyridazin-5-one PoSSuM
UNX Unknown atom or ion PoSSuM
FBP 1,6-di-O-phosphono-beta-D-fructofuranose PoSSuM
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Structure summary

Code : 3U2Z   PDBj   RCSB PDB   PDBe
Header : transferase/transferase activator
Title : Activator-Bound Structure of Human Pyruvate Kinase M2
Release Data : 2012-09-12
Compound :
mol_id molecule chains synonym
1 Pyruvate kinase isozymes M1/M2 A,B,C,D Cytosolic thyroid hormone-binding protein, CTHBP, Opa-interacting protein 3, OIP-3, Pyruvate kinase 2/3, Pyruvate kinase muscle isozyme, Thyroid hormone-binding protein 1, THBP1, Tumor M2-PK, p58
ec: 2.7.1.40
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Escherichia coli  (taxid:562)
gene: PKM2, OIP3, PK2, PK3, PKM
expression_system_strain: DE3
expression_system_vector_type: plasmid
expression_system_plasmid: pET28a-LIC
Authors : Hong, B., Dimov, S., Tempel, W., Auld, D., Thomas, C., Boxer, M., Jianq, J.-K., Skoumbourdis, A., Min, S., Southall, N., Arrowsmith, C.H., Edwards, A.M., Bountra, C., Weigelt, J., Inglese, J., Park, H., Structural Genomics Consortium (SGC)
Keywords : transferase, Structural Genomics, Structural Genomics Consortium, SGC, transferase-transferase activator complex
Exp. method : X-RAY DIFFRACTION ( 2.1000 Å )
Citation :

Pyruvate kinase M2 activators promote tetramer formation and suppress tumorigenesis.

Anastasiou, D.,Yu, Y.,Israelsen, W.J.  et al.
(2012)  Nat.Chem.Biol.  8 : 839 - 847

PubMed: 22922757
DOI: 10.1038/nchembio.1060

Reaction

Chain : A, B, C, D
UniProt : P14618 (KPYM_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
[Isoform M2]
ATP + pyruvate = ADP + H(+) + phosphoenolpyruvate 		2.7.1.40 PubMed:15996096, PubMed:1854723, PubMed:20847263
right-to-left PubMed:15996096, PubMed:1854723, PubMed:20847263
[Isoform M2]
ATP + L-tyrosyl-[protein] = ADP + H(+) + O-phospho-L-tyrosyl- [protein] 		2.7.10.2 PubMed:22306293, PubMed:24120661
left-to-right
[Isoform M2]
ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L- threonyl-[protein] 		2.7.11.1 PubMed:22901803, PubMed:24120661
left-to-right PubMed:22901803, PubMed:24120661
[Isoform M1]
ATP + pyruvate = ADP + H(+) + phosphoenolpyruvate 		2.7.1.40 PubMed:20847263
-