Brand  (β version)

PDB ID: 3TYK

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Ligands
Code Name Style Show Link
CL Chloride ion PoSSuM
HY0 Hygromycin B variant PoSSuM
Non-standard Residues
Code Name Show
CSX S-oxy cysteine
MSE Selenomethionine
Glycosylation
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Structure summary

Code : 3TYK   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE/ANTIBIOTIC
Title : Crystal structure of aminoglycoside phosphotransferase APH(4)-Ia
Release Data : 2011-10-12
Compound :
mol_id molecule chains synonym
1 Hygromycin-B 4-O-kinase A APH(4), Hygromycin B phosphotransferase, Hygromycin-B kinase
ec: 2.7.1.163
Source :
mol_id organism_scientific expression_system
1 Escherichia coli  (taxid:562) Escherichia coli  (taxid:469008)
strain: Plasmid pKC222
gene: APH(4), hph
expression_system_strain: BL21(DE3)
expression_system_vector_type: plasmid
expression_system_plasmid: P15TV-L
Authors : Stogios, P.J., Shabalin, I.G., Shakya, T., Evdokmova, E., Fan, Y., Chruszcz, M., Minor, W., Wright, G.D., Savchenko, A., Anderson, W.F., Midwest Center for Structural Genomics (MCSG)
Keywords : Midwest Center for Structural Genomics, MCSG, PSI-2, AMINOGLYCOSIDE PHOSPHOTRANSFERASE, EUKARYOTIC PROTEIN KINASE-LIKE FOLD, PHOSPHOTRANSFERASE/KINASE, ANTIBIOTIC RESISTANCE, AMINOGLYCOSIDE BINDING, PHOSPHORYLATION, TRANSFERASE-ANTIBIOTIC COMPLEX, cytoplasmic, Protein Structure Initiative
Exp. method : X-RAY DIFFRACTION ( 1.95 Å )
Citation :

Structure and function of APH(4)-Ia, a hygromycin B resistance enzyme.

Stogios, P.J.,Shakya, T.,Evdokimova, E.  et al.
(2011)  J.Biol.Chem.  286 : 1966 - 1975

PubMed: 21084294

Reaction

Chain : A
UniProt : P00557 (KHYB_ECOLX)
Reaction: EC: Evidence:
Physiological Direction:
ATP + hygromycin B = 4-O-phosphohygromycin B + ADP + H(+) 2.7.1.163 PubMed:6318654
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