Brand  (β version)

PDB ID: 3T8N

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Ligands
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SO4 Sulfate ion PoSSuM
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Structure summary

Code : 3T8N   PDBj   RCSB PDB   PDBe
Header : ISOMERASE
Title : Crystal structure of ketosteroid isomerase Y16AD103A from Pseudomonas putida
Release Data : 2011-11-23
Compound :
mol_id molecule chains synonym
1 Steroid Delta-isomerase A,B,D,F Delta(5)-3-ketosteroid isomerase
ec: 5.3.3.1
mutation: Y16A, D103A
Source :
mol_id organism_scientific organism_common expression_system
1 Pseudomonas putida  (taxid:303) Arthrobacter siderocapsulatus Escherichia coli  (taxid:469008)
gene: ksi
expression_system_strain: BL21(DE3)
expression_system_vector_type: plasmid
expression_system_plasmid: pET21
Authors : Gonzalez, A., Tsai, Y., Schwans, J., Sunden, F., Herschlag, D.
Keywords : Isomerase
Exp. method : X-RAY DIFFRACTION ( 1.47 Å )
Citation :

Evaluating the catalytic contribution from the oxyanion hole in ketosteroid isomerase.

Schwans, J.P.,Sunden, F.,Gonzalez, A.  et al.
(2011)  J.Am.Chem.Soc.  133 : 20052 - 20055

PubMed: 22053826
DOI: 10.1021/ja208050t

Reaction

Chain : A, B, D, F
UniProt : P07445 (SDIS_PSEPU)
Reaction: EC: Evidence:
Physiological Direction:
a 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid 5.3.3.1 -
-