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Ligands
Code Name Style Show Link
ZN Zinc ion
SO4 Sulfate ion
MES 2-(N-morpholino)-ethanesulfonic acid
BGC Beta-D-glucopyranose
: Polysaccharide
Non-standard Residues
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Glycosylation
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Code : 3SCU   PDBj   RCSB PDB   PDBe
Header : HYDROLASE
Title : Crystal Structure of Rice BGlu1 E386G Mutant Complexed with Cellopentaose
Release Data : 2012-02-15
Compound :
mol_id molecule chains synonym
1 Beta-glucosidase 7 A,B Os3bglu7
ec: 3.2.1.21
mutation: E386G
Source :
mol_id organism_scientific organism_common expression_system
1 Oryza sativa Japonica Group  (taxid:39947) Japanese rice Escherichia coli  (taxid:562)
strain: ORION
gene: BGLU1, BGLU7, LOC_Os03g49600, Os03g0703000, os3bglu7, OSJNBa0004L11.16
expression_system_strain: Origami(DE3)
expression_system_vector_type: Plasmid
expression_system_plasmid: pET32a(+)
Authors : Pengthaisong, S., Withers, S.G., Kuaprasert, B., Ketudat Cairns, J.R.
Keywords : BETA-ALPHA-BARRELS, GLYCOSYNTHASE, OLIGOSACCHARIDE SYNTHESIS, TRANSGLUCOSYLATION, HYDROLASE
Exp. method : X-RAY DIFFRACTION ( 1.58 Å )
Citation :

Structural investigation of the basis for cellooligosaccharide synthesis by rice BGlu1 glycosynthases

Pengthaisong, S.,Withers, S.G.,Kuaprasert, B.  et al.
to be published 

Chain : A, B
UniProt : Q75I93 (BGL07_ORYSJ)
Reaction: EC: Evidence:
Physiological Direction:
Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose. 3.2.1.21 PubMed:14692878
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