Brand  (β version)

PDB ID: 3SC0

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Ligands
Code Name Style Show Link
COB Co-methylcobalamin PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 3SC0   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : Crystal Structure of MMACHC (1-238), a human B12 processing enzyme, complexed with MethylCobalamin
Release Data : 2011-06-22
Compound :
mol_id molecule chains
1 Methylmalonic aciduria and homocystinuria type C protein A
fragment: B12 binding domain, Residues 1-238
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Escherichia coli  (taxid:562)
gene: MMACHC
expression_system_strain: BL21
expression_system_vector_type: plasmid
expression_system_plasmid: pMCSG7
Authors : Koutmos, M., Gherasim, C., Smith, J.L., Banerjee, R.
Keywords : MMACHC, CblC, Cobalamin, Flavin, Glutathione, Flavin Reductase, Oxidoreductase, Maturase
Exp. method : X-RAY DIFFRACTION ( 1.9500 Å )
Citation :

Structural basis of multifunctionality in a vitamin B12-processing enzyme.

Koutmos, M.,Gherasim, C.,Smith, J.L.  et al.
(2011)  J.Biol.Chem.  286 : 29780 - 29787

PubMed: 21697092
DOI: 10.1074/jbc.M111.261370

Reaction

Chain : A
UniProt : Q9Y4U1 (MMAC_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
2 cob(II)alamin-[cyanocobalamin reductase] + 2 hydrogen cyanide + NADP(+) = 2 apo-[cyanocobalamin reductase] + 2 cyanocob(III)alamin + H(+) + NADPH 1.16.1.6 PubMed:18779575, PubMed:19700356, PubMed:21697092, PubMed:25809485
right-to-left PubMed:19700356
an R-cob(III)alamin + apo-[alkylcobalamin reductase] + glutathione = an S-substituted glutathione + cob(I)alamin- [alkylcobalamin reductase] + H(+) 2.5.1.151 PubMed:19801555, PubMed:21697092, PubMed:22642810, PubMed:25809485
left-to-right PubMed:25809485
apo-[alkylcobalamin reductase] + glutathione + methylcob(III)alamin = cob(I)alamin-[alkylcobalamin reductase] + H(+) + S-methyl glutathione 2.5.1.151 PubMed:19801555, PubMed:22642810, PubMed:25809485
left-to-right PubMed:25809485
adenosylcob(III)alamin + apo-[alkylcobalamin reductase] + glutathione = cob(I)alamin-[alkylcobalamin reductase] + H(+) + S- adenosylglutathione 2.5.1.151 PubMed:19801555
left-to-right