Brand  (β version)

PDB ID: 3SAW

Hetero Atom Contents

  The number of atoms exceeds 100,000.
  So, it can not be displayed here.

Select unit:

Select hetatm:   

close
information
centroid:
interaction residue:

Select chain:   Sequence  

Data format:   

Color scheme of protein:

Ligands
Code Name Style Show Link
ZN Zinc ion PoSSuM
Non-standard Residues
Code Name Show
CX2 2'-deoxy-5'-O-{(R)-hydroxy[(2-sulfanylethyl)amino]phosphoryl}cytidine
Glycosylation
Code Name Emphasize
Modification
Code Name Show

Structure summary

Code : 3SAW   PDBj   RCSB PDB   PDBe
Header : HYDROLASE/DNA
Title : MUTM Slanted complex 8 with R112A mutation
Release Data : 2012-01-11
Compound :
mol_id molecule chains
1 DNA GLYCOSYLASE A
ec: 4.2.99.18
mol_id molecule chains
2 5'-D(*A*GP*GP*TP*AP*GP*AP*CP*AP*AP*GP*GP*AP*CP*GP*C)-3' B
mol_id molecule chains
3 5'-D(*TP*GP*CP*GP*TP*C*CP*TP*TP*GP*TP*(CX2)P*TP*AP*CP*C)-3' C
Source :
mol_id organism_scientific organism_common expression_system
1 Geobacillus stearothermophilus  (taxid:1422) Bacillus stearothermophilus Escherichia coli  (taxid:469008)
gene: MUTM
expression_system_strain: BL21(DE3) PLYSS
expression_system_vector_type: plasmid
expression_system_plasmid: pET24B
mol_id organism_scientific
2
synthetic: yes
mol_id organism_scientific
3
synthetic: yes
Authors : Qi, Y., Verdine, G.L.
Keywords : DNA GLYCOSYLASE, DNA REPAIR, DAMAGE SEARCH, TRANSLOCATION, DISULFIDE CROSSLINKING, DNA DAMAGE, DNA-BINDING, GLYCOSIDASE, HYDROLASE, LYASE, METAL-BINDING, MULTIFUNCTIONAL ENZYME, ZINC-FINGER, HYDROLASE-DNA complex
Exp. method : X-RAY DIFFRACTION ( 2.35 Å )
Citation :

Strandwise translocation of a DNA glycosylase on undamaged DNA.

Qi, Y.,Nam, K.,Spong, M.C.  et al.
(2012)  Proc.Natl.Acad.Sci.USA  109 : 1086 - 1091

PubMed: 22219368
DOI: 10.1073/pnas.1111237108

Reaction

Chain : A
UniProt : P84131 (P84131_GEOSE)
Reaction: EC: Evidence:
Physiological Direction:
2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'- deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3- dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-monophospho- 2'-deoxyribonucleoside-DNA + H(+) 4.2.99.18 ARBA:ARBA00024490, HAMAP- Rule:MF_00103
-
Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N- methyl)formamidopyrimidine. 3.2.2.23 ARBA:ARBA00001668, HAMAP- Rule:MF_00103
-