Brand  (β version)

PDB ID: 3SAR

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Ligands
Code Name Style Show Link
GOL Glycerol PoSSuM
ZN Zinc ion PoSSuM
Non-standard Residues
Code Name Show
CX2 2'-deoxy-5'-O-{(R)-hydroxy[(2-sulfanylethyl)amino]phosphoryl}cytidine
Glycosylation
Code Name Emphasize
Modification
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Structure summary

Code : 3SAR   PDBj   RCSB PDB   PDBe
Header : HYDROLASE/DNA
Title : MUTM Slanted complex 1
Release Data : 2012-01-11
Compound :
mol_id molecule chains
1 Formamidopyrimidine-DNA glycosylase A
mutation: Q166C
mol_id molecule chains
2 5'-D(*AP*GP*GP*TP*AP*GP*AP*CP*CP*TP*GP*GP*AP*CP*GP*C)-3' C
mol_id molecule chains
3 5'-D(*TP*GP*C*GP*TP*CP*CP*AP*GP*GP*TP*(CX2) P*TP*AP*CP*C)-3' D
Source :
mol_id organism_scientific expression_system
1 Geobacillus stearothermophilus  (taxid:1422) Escherichia coli  (taxid:469008)
gene: mutM
expression_system_strain: BL21(DE3) PLYSS
expression_system_vector_type: plasmid
expression_system_plasmid: pET24B
mol_id organism_scientific
2
synthetic: yes
other_details: SYNTHETIC DNA
mol_id organism_scientific
3
synthetic: yes
other_details: SYNTHETIC DNA
Authors : Banerjee, A., Qi, Y., Spong, M.C., Verdine, G.L.
Keywords : DNA GLYCOSYLASE, DNA REPAIR, DAMAGE SEARCH, TRANSLOCATION, DISULFIDE CROSSLINKING, DNA DAMAGE, DNA-BINDING, GLYCOSIDASE, HYDROLASE, LYASE, METAL-BINDING, MULTIFUNCTIONAL ENZYME, ZINC-FINGER, HYDROLASE-DNA complex
Exp. method : X-RAY DIFFRACTION ( 1.95 Å )
Citation :

Strandwise translocation of a DNA glycosylase on undamaged DNA.

Qi, Y.,Nam, K.,Spong, M.C.  et al.
(2012)  Proc.Natl.Acad.Sci.USA  109 : 1086 - 1091

PubMed: 22219368
DOI: 10.1073/pnas.1111237108

Reaction

Chain : A
UniProt : P84131 (P84131_GEOSE)
Reaction: EC: Evidence:
Physiological Direction:
2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'- deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3- dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho-2'- deoxyribonucleoside-DNA + H(+) 4.2.99.18 ARBA:ARBA00044632, HAMAP-Rule:MF_00103
-
Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N- methyl)formamidopyrimidine. 3.2.2.23 ARBA:ARBA00001668, HAMAP-Rule:MF_00103
-
Cofactor: Evidence: Note:
Zn(2+) ECO:0000256 | HAMAP-Rule:MF_00103
Binds 1 zinc ion per subunit.