Brand  (β version)

PDB ID: 3QTC

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Ligands
Code Name Style Show Link
0A1 O-methyl-L-tyrosine PoSSuM
ANP Phosphoaminophosphonic acid-adenylate ester PoSSuM
EDO 1,2-ethanediol PoSSuM
MG Magnesium ion PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 3QTC   PDBj   RCSB PDB   PDBe
Header : LIGASE
Title : Crystal structure of the catalytic domain of MmOmeRS, an O-methyl tyrosyl-tRNA synthetase evolved from Methanosarcina mazei PylRS, complexed with O-methyl tyrosine and AMP-PNP
Release Data : 2011-05-25
Compound :
mol_id molecule chains synonym
1 Pyrrolysyl-tRNA synthetase A Pyrrolysine-tRNA ligase, PylRS
ec: 6.1.1.26
fragment: catalytic domain of PylRS, UNP residues 185-454
mutation: A302T, N346V, C348W, Y384F, V401L
Source :
mol_id organism_scientific organism_common expression_system
1 Methanosarcina mazei  (taxid:2209) Methanosarcina frisia Escherichia coli  (taxid:469008)
gene: MM_1445, pylS
expression_system_strain: BL21(DE3)
expression_system_vector_type: plasmid
expression_system_plasmid: pet28a
Authors : Dellas, N., Takimoto, J.K., Noel, J.P., Wang, L.
Keywords : pyrrolysyl-tRNA synthetase, aminoacyl-tRNA synthetase, ATP binding, O-methyl tyrosine binding, magnesium binding, aminoacylation, esterification, LIGASE
Exp. method : X-RAY DIFFRACTION ( 1.7500 Å )
Citation :

Stereochemical Basis for Engineered Pyrrolysyl-tRNA Synthetase and the Efficient in Vivo Incorporation of Structurally Divergent Non-native Amino Acids.

Takimoto, J.K.,Dellas, N.,Noel, J.P.  et al.
(2011)  Acs Chem.Biol.  6 : 733 - 743

PubMed: 21545173
DOI: 10.1021/cb200057a

Reaction

Chain : A
UniProt : Q8PWY1 (PYLS_METMA)
Reaction: EC: Evidence:
Physiological Direction:
ATP + L-pyrrolysine + tRNA(Pyl) = AMP + diphosphate + L- pyrrolysyl-tRNA(Pyl) 6.1.1.26 HAMAP- Rule:MF_01573
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