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Ligands
Code Name Style Show Link
CP6 5-(4-chloro-phenyl)-6-ethyl-pyrimidine-2,4-diamine
NDP Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate
UMP 2'-deoxyuridine 5'-monophosphate
Non-standard Residues
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Code : 3QGT   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE, TRANSFERASE/INHIBITOR
Title : Crystal structure of Wild-type PfDHFR-TS COMPLEXED WITH NADPH, dUMP AND PYRIMETHAMINE
Release Data : 2011-06-29
Compound :
mol_id molecule chains
1 Bifunctional dihydrofolate reductase-thymidylate synthase A,B
ec: 1.5.1.3, 2.1.1.45
Source :
mol_id organism_scientific expression_system
1 Plasmodium falciparum  (taxid:5833) Escherichia coli  (taxid:562)
strain: TM4
gene: DHFR-TS
expression_system_strain: BL21(DE3)
expression_system_vector_type: plasmid
expression_system_plasmid: pET17b
Authors : Chitnumsub, P., Yuthavong, Y.
Keywords : malarial DHFR-TS antifolate complex, rossmann fold, reductase, Dihydrofolate binding, dUMP binding, NADPH binding, OXIDOREDUCTASE, TRANSFERASE-INHIBITOR complex
Exp. method : X-RAY DIFFRACTION ( 2.3000 Å )
Citation :

Trypanosomal dihydrofolate reductase reveals natural antifolate resistance

Vanichtanankul, J.,Taweechai, S.,Yuvaniyama, J.  et al.
(2011)  Acs Chem.Biol.  6 : 905 - 911

PubMed: 21650210
DOI: 10.1021/cb200124r

Chain : A, B
UniProt : A7UD81 (A7UD81_PLAFA)
Reaction: EC: Evidence:
Physiological Direction:
(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP = 7,8- dihydrofolate + dTMP 2.1.1.45 ARBA:ARBA00001707
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(6S)-5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + H(+) + NADPH 1.5.1.3 ARBA:ARBA00001315
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