Brand  (β version)

PDB ID: 3Q24

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Ligands
Code Name Style Show Link
GTP Guanosine-5'-triphosphate PoSSuM
5GP Guanosine-5'-monophosphate PoSSuM
2HP Dihydrogenphosphate ion PoSSuM
POP Pyrophosphate 2- PoSSuM
Non-standard Residues
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Glycosylation
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Modification
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Structure summary

Code : 3Q24   PDBj   RCSB PDB   PDBe
Header : TRANSFERASE/DNA/RNA
Title : X-ray crystal structure of the N4 mini-VRNAP and P2_7a promoter transcription initiation complex with pppGpG and pyrophosphate: product complex
Release Data : 2011-02-16
Compound :
mol_id molecule chains
1 Virion RNA polymerase A,B
ec: 2.7.7.6
mol_id molecule chains
2 DNA (5'-D(*TP*GP*CP*CP*TP*CP*CP*CP*AP*GP*GP*CP*AP*TP*CP*CP*AP*AP*AP*AP*GP*AP*AP*GP*CP*GP*GP*AP*GP*CP*TP*TP*CP*TP*TP*C)-3') C,D
Source :
mol_id organism_scientific organism_common expression_system
1 Enterobacteria phage N4  (taxid:10752) Bacteriophage N4 Escherichia coli  (taxid:562)
gene: gp50
expression_system_strain: BL21
expression_system_vector_type: plasmid
expression_system_plasmid: PKMK56
mol_id organism_scientific
2 Synthetic construct  (taxid:32630)
synthetic: yes
Authors : Gleghorn, M.L., Murakami, K.S.
Keywords : PROTEIN-DNA COMPLEX, TWO-METAL CATALYSIS, DE NOVO TRANSCRIPTION INITIATION, NUCLEOTIDYLTRANSFERASE, INITIATION COMPLEX, DNA-HAIRPIN, VIRION RNA POLYMERASE, TRANSFERASE-DNA-RNA COMPLEX
Exp. method : X-RAY DIFFRACTION ( 1.81 Å )
Citation :

X-ray crystal structures elucidate the nucleotidyl transfer reaction of transcript initiation using two nucleotides.

Gleghorn, M.L.,Davydova, E.K.,Basu, R.  et al.
(2011)  Proc.Natl.Acad.Sci.USA  108 : 3566 - 3571

PubMed: 21321236
DOI: 10.1073/pnas.1016691108

Reaction

Chain : A, B
UniProt : Q859P9 (RPOLV_BPN4)
Reaction: EC: Evidence:
Physiological Direction:
RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate 2.7.7.6 PubMed:21321236
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Cofactor: Evidence: Note:
Mg(2+) ECO:0000269 | PubMed:21321236
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