Brand  (β version)

PDB ID: 3P5R

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Ligands
Code Name Style Show Link
MG Magnesium ion PoSSuM
FGG (2z,6e,10e)-2-fluoro-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraen-1-yl trihydrogen diphosphate PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 3P5R   PDBj   RCSB PDB   PDBe
Header : LYASE
Title : Crystal Structure of Taxadiene Synthase from Pacific Yew (Taxus brevifolia) in complex with Mg2+ and 2-fluorogeranylgeranyl diphosphate
Release Data : 2010-12-29
Compound :
mol_id molecule chains synonym
1 Taxadiene synthase A,B Taxa-4(5),11(12)-diene synthase
ec: 4.2.3.17
fragment: UNP residues 108-862
Source :
mol_id organism_scientific organism_common expression_system
1 Taxus brevifolia  (taxid:46220) Pacific yew Escherichia coli  (taxid:562)
tissue: stem
gene: TDC1
expression_system_strain: BL21(DE3) CodonPlus RIL
expression_system_vector_type: Plasmid
expression_system_plasmid: pET22bTV
Authors : Koksal, M., Christianson, D.W.
Keywords : class I and II terpene cyclase fold, diterpene cyclase, DDXXD motif, NSE/DTE motif, 2-fluoro-geranylgeranyl diphosphate, Biosynthesis of paclitaxel, LYASE
Exp. method : X-RAY DIFFRACTION ( 2.247 Å )
Citation :

Taxadiene synthase structure and evolution of modular architecture in terpene biosynthesis.

Koksal, M.,Jin, Y.,Coates, R.M.  et al.
(2011)  Nature  469 : 116 - 120

PubMed: 21160477
DOI: 10.1038/nature09628

Reaction

Chain : A, B
UniProt : Q41594 (TASY_TAXBR)
Reaction: EC: Evidence:
Physiological Direction:
(2E,6E,10E)-geranylgeranyl diphosphate = taxa-4(5),11(12)- diene + diphosphate 4.2.3.17 -
-
Cofactor: Evidence: Note:
Mg(2+) ECO:0000250
Binds 3 Mg(2+) ions per subunit.