Brand  (β version)

PDB ID: 3OSQ

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Ligands
Code Name Style Show Link
SO4 Sulfate ion PoSSuM
GLC Alpha-D-glucopyranose PoSSuM
: Polysaccharide
Non-standard Residues
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C12 2-(1-amino-2-hydroxypropyl)-4-(4-hydroxybenzyl)-1-(2-oxoethyl)-1h-imidazol-5-olate
Glycosylation
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Structure summary

Code : 3OSQ   PDBj   RCSB PDB   PDBe
Header : FLUORESCENT PROTEIN, Transport Protein
Title : Maltose-bound maltose sensor engineered by insertion of circularly permuted green fluorescent protein into E. coli maltose binding protein at position 175
Release Data : 2011-10-26
Compound :
mol_id molecule chains synonym
1 Maltose-binding periplasmic protein,Green fluorescent protein A MBP,MMBP,Maltodextrin-binding protein
fragment: GFP P42212 residues 2-146, 147-238, MBP P0AEX9 residues 27-199, 201-396
Source :
mol_id organism_scientific organism_common expression_system
1 Escherichia coli O157:H7  (taxid:83334) Jellyfish Escherichia coli  (taxid:469008)
gene: malE, Z5632, ECs5017
expression_system_strain: BL21(DE3)
expression_system_vector_type: plasmid
expression_system_plasmid: pRSET
Authors : Echevarria, I.M., Marvin, J.S., Looger, L.L., Schreiter, E.R.
Keywords : Engineered Protein, Sensor Protein, Fluorescent Protein, MBP, GFP, Maltose Sensor, Transport Protein
Exp. method : X-RAY DIFFRACTION ( 1.90 Å )
Citation :

A genetically encoded, high-signal-to-noise maltose sensor.

Marvin, J.S.,Schreiter, E.R.,Echevarria, I.M.  et al.
(2011)  Proteins  79 : 3025 - 3036

PubMed: 21989929
DOI: 10.1002/prot.23118

Reaction

Chain : A
UniProt : P0AEY0 (MALE_ECO57)
Reaction : -
Chain : A
UniProt : P42212 (GFP_AEQVI)
Reaction : -