Brand  (β version)

PDB ID: 3N2V

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Ligands
Code Name Style Show Link
CA Calcium ion PoSSuM
JT5 N~2~-(biphenyl-4-ylsulfonyl)-N-hydroxy-N~2~-(2-hydroxyethyl)glycinamide PoSSuM
ZN Zinc ion PoSSuM
Non-standard Residues
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Structure summary

Code : 3N2V   PDBj   RCSB PDB   PDBe
Header : Hydrolase/Hydrolase inhibitor
Title : Crystal structure of the catalytic domain of human MMP12 complexed with the inhibitor N-hydroxy-2-(N-hydroxyethyl)biphenyl-4-ylsulfonamido)acetamide
Release Data : 2010-11-10
Compound :
mol_id molecule chains synonym
1 Macrophage metalloelastase A MME, Matrix metalloproteinase-12, MMP-12, Macrophage elastase, ME, hME
ec: 3.4.24.65
fragment: catalytic domain
mutation: F171D
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human Escherichia coli  (taxid:562)
gene: MMP12, HME
expression_system_strain: BL21
expression_system_vector: plasmid
expression_system_plasmid: PET21
Authors : Calderone, V.
Keywords : MATRIX METALLOPROTEINASE, MMP12, ELASTASE, COMPLEX (ELASTASE-INHIBITOR), METALLO ELASTASE, EXTRACELLULAR MATRIX, GLYCOPROTEIN, HYDROLASE, METAL-INDING, METALLOPROTEASE, PROTEASE, SECRETED, ZYMOGEN, Hydrolase-Hydrolase inhibitor complex
Exp. method : X-RAY DIFFRACTION ( 1.55 Å )
Citation :

Structure-based approach to nanomolar, water soluble matrix metalloproteinases inhibitors (MMPIs).

Attolino, E.,Calderone, V.,Dragoni, E.  et al.
(2010)  Eur.J.Med.Chem.  45 : 5919 - 5925

PubMed: 20965620
DOI: 10.1016/j.ejmech.2010.09.057

Exploring the subtleties of drug-receptor interactions: the case of matrix metalloproteinases.

Bertini, I.,Calderone, V.,Fragai, M.  et al.
(2007)  J.Am.Chem.Soc.  129 : 2466 - 2475

PubMed: 17269766
DOI: 10.1021/ja065156z

Reaction

Chain : A
UniProt : P39900 (MMP12_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
Hydrolysis of soluble and insoluble elastin. Specific cleavages are also produced at 14-Ala-|-Leu-15 and 16-Tyr-|-Leu-17 in the B chain of insulin. 3.4.24.65 -
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