Brand  (β version)

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information
centroid:
interaction residue:

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Ligands
Code Name Link
C1C 1,1'-binaphthalene-2,2'-dicarboxylic acid
GOL Glycerol
SO4 Sulfate ion
Code : 3MKS   PDBj   RCSB PDB   PDBe
Header : LIGASE/CELL CYCLE
Title : Crystal Structure of yeast Cdc4/Skp1 in complex with an allosteric inhibitor SCF-I2
Release Data : 2010-07-21
Compound :
mol_id molecule chains synonym
1 Suppressor of kinetochore protein 1 A,C Centromere DNA-binding protein complex CBF3 subunit D, E3 ubiquitin ligase complex SCF subunit SKP1
fragment: UNP Residues 2-194 with 37-64 deleted
mol_id molecule chains synonym
2 Cell division control protein 4 B,D F-box protein CDC4, E3 ubiquitin ligase complex SCF subunit CDC4
fragment: UNP Residues 263-744 with 602-605 and 609-624 deleted
Source :
mol_id organism_scientific organism_common expression_system
1 Saccharomyces cerevisiae  (taxid:4932) Brewer's yeast,lager beer yeast,yeast Escherichia coli  (taxid:469008)
gene: SKP1, CBF3D, YDR328C, D9798.14
expression_system_strain: BL21(DE3)codon plus
expression_system_vector_type: plasmid
expression_system_plasmid: pProEx
mol_id organism_scientific organism_common expression_system
2 Saccharomyces cerevisiae  (taxid:4932) Brewer's yeast,lager beer yeast,yeast Escherichia coli  (taxid:469008)
gene: CDC4, YFL009W
expression_system_strain: BL21(DE3)codon plus
expression_system_vector_type: plasmid
expression_system_plasmid: pProEx
Authors : Orlicky, S., Sicheri, F., Tyers, M., Tang, X.
Keywords : ubiquitin ligase, protein binding, small molecule complex, LIGASE/CELL CYCLE, LIGASE-CELL CYCLE complex
Exp. method : X-RAY DIFFRACTION ( 2.60 Å )
Citation :

An allosteric inhibitor of substrate recognition by the SCF(Cdc4) ubiquitin ligase.

Orlicky, S.,Tang, X.,Neduva, V.  et al.
(2010)  Nat.Biotechnol.  28 : 733 - 737

PubMed: 20581844
DOI: 10.1038/nbt.1646

Chain : A, C
UniProt : P52286 (SKP1_YEAST)
Reaction : -
Chain : B, D
UniProt : P07834 (CDC4_YEAST)
Reaction : -