Brand  (β version)

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Ligands
Code Name Link
BME Beta-mercaptoethanol
CAQ Catechol
CL Chloride ion
FE Fe (III) ion
GOL Glycerol
SO4 Sulfate ion
Code : 3MI5
Header : OXIDOREDUCTASE
Title : Axial Ligand Swapping In Double Mutant Maintains Intradiol-cleavage Chemistry in Protocatechuate 3,4-Dioxygenase
Release Data : 2011-04-27
Compound :
mol_id molecule chains synonym
1 Protocatechuate 3,4-dioxygenase alpha chain A,B,C,D,E,F 3,4-PCD
ec: 1.13.11.3
mol_id molecule chains synonym
2 Protocatechuate 3,4-dioxygenase beta chain M,N,O,P,Q,R 3,4-PCD
ec: 1.13.11.3
mutation: Y148H/H163Y
Source :
mol_id organism_scientific expression_system
1 Pseudomonas putida  (taxid:303) Escherichia coli  (taxid:469008)
strain: P. putida
gene: pcaG
expression_system_strain: BL21 (DE3)
expression_system_vector_type: plasmid
expression_system_plasmid: pCE vector, pT7-7
mol_id organism_scientific expression_system
2 Pseudomonas putida  (taxid:303) Escherichia coli  (taxid:469008)
gene: pcaH
expression_system_strain: BL21 (DE3)
expression_system_vector_type: plasmid
expression_system_plasmid: pCE vector, pT7-7
Authors : Purpero, V.M., Lipscomb, J.D.
Keywords : dioxygenase, non-heme, iron, intradiol, catechol, substrate analogue, protocatechuate, OXIDOREDUCTASE
Exp. method : X-RAY DIFFRACTION ( 1.780 Å )
Citation :

Axial Ligand Swapping In Double Mutant Maintains Intradiol-cleavage Chemistry in Protocatechuate 3,4-Dioxygenase

Purpero, V.M.,Lipscomb, J.D.
To be Published 

Chain : A, B, C, D, E, F
UniProt : P00436 (PCXA_PSEPU)
Reaction : 3,4-dihydroxybenzoate + O(2) = 3-carboxy- cis,cis-muconate.
Chain : M, N, O, P, Q, R
UniProt : P00437 (PCXB_PSEPU)
Reaction : 3,4-dihydroxybenzoate + O(2) = 3-carboxy- cis,cis-muconate.