Brand  (β version)

PDB ID: 3MAT

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Ligands
Code Name Style Show Link
CO Cobalt (II) ion PoSSuM
NA Sodium ion PoSSuM
Non-standard Residues
Code Name Show
0A9 Methyl L-phenylalaninate
AHH Amino-hydroxyheptanoic acid
Glycosylation
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Modification
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Structure summary

Code : 3MAT   PDBj   RCSB PDB   PDBe
Header : HYDROLASE/HYDROLASE INHIBITOR
Title : E.COLI METHIONINE AMINOPEPTIDASE TRANSITION-STATE INHIBITOR COMPLEX
Release Data : 1999-06-18
Compound :
mol_id molecule chains
1 METHIONINE AMINOPEPTIDASE A
ec: 3.4.11.18
mutation: R175Q
mol_id molecule chains
2 bestatin-based inhibitor (3R)-amino-(2S)-hydroxyheptanoyl-l-Ala-l-Leu-l-Val-l-Phe-OMe I
other_details: SYNTHETIC
Source :
mol_id organism_scientific expression_system
1 Escherichia coli  (taxid:562) Escherichia coli  (taxid:562)
expression_system_cell_line: BL21(DE3)
expression_system_plasmid: PET28B
mol_id organism_scientific
2
synthetic: yes
Authors : Lowther, W.T., Orville, A.M., Madden, D.T., Lim, S., Rich, D.H., Matthews, B.W.
Keywords : ALPHA-AMINOACYLPEPTIDE, TRANSITION-STATE ANALOG, HYDROLASE-HYDROLASE INHIBITOR complex
Exp. method : X-RAY DIFFRACTION ( 2.0 Å )
Citation :

Escherichia coli methionine aminopeptidase: implications of crystallographic analyses of the native, mutant, and inhibited enzymes for the mechanism of catalysis.

Lowther, W.T.,Orville, A.M.,Madden, D.T.  et al.
(1999)  Biochemistry  38 : 7678 - 7688

PubMed: 10387007
DOI: 10.1021/bi990684r

The anti-angiogenic agent fumagillin covalently modifies a conserved active-site histidine in the Escherichia coli methionine aminopeptidase.

Lowther, W.T.,McMillen, D.A.,Orville, A.M.  et al.
(1998)  Proc.Natl.Acad.Sci.USA  95 : 12153 - 12157

PubMed: 9770455
DOI: 10.1073/pnas.95.21.12153

Structure of the cobalt-dependent methionine aminopeptidase from Escherichia coli: a new type of proteolytic enzyme.

Roderick, S.L.,Matthews, B.W.
(1993)  Biochemistry  32 : 3907 - 3912

PubMed: 8471602
DOI: 10.1021/bi00066a009

Reaction

Chain : A
UniProt : P07906 (multiple entry names are found)
Reaction : -