Brand  (β version)

PDB ID: 3LZ9

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Ligands
Code Name Style Show Link
FPF (2z,6e)-2-fluoro-3,7,11-trimethyldodeca-2,6,10-trien-1-yl trihydrogen diphosphate PoSSuM
MG Magnesium ion PoSSuM
Non-standard Residues
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Structure summary

Code : 3LZ9   PDBj   RCSB PDB   PDBe
Header : LYASE
Title : The Crystal Structure of 5-epi-aristolochene synthase M4 mutant complexed with (2-trans,6-trans)-2-fluorofarnesyl diphosphate
Release Data : 2010-04-07
Compound :
mol_id molecule chains synonym
1 Aristolochene synthase A 5-epi-aristolochene synthase, EAS
ec: 4.2.3.9
mutation: A274T, V372I, Y406L, V516I
Source :
mol_id organism_scientific organism_common expression_system
1 Nicotiana tabacum  (taxid:4097) American tobacco,tobacco Escherichia coli  (taxid:562)
gene: EAS3, EAS4
expression_system_strain: BL21(DE3)
expression_system_vector_type: plasmid
expression_system_plasmid: pHis9GW
Authors : Noel, J.P., Dellas, N., Faraldos, J.A., Zhao, M., Hess Jr., B.A., Smentek, L., Coates, R.M., O'Maille, P.E.
Keywords : plant terpenoid cyclase, 5-epi-aristolochene synthase, lyase, metal-binding domain, (2-trans, 6-trans)-2-fluorofarnesyl diphosphate, Cytoplasm, Magnesium, Metal-binding
Exp. method : X-RAY DIFFRACTION ( 2.280 Å )
Citation :

Structural elucidation of cisoid and transoid cyclization pathways of a sesquiterpene synthase using 2-fluorofarnesyl diphosphates.

Noel, J.P.,Dellas, N.,Faraldos, J.A.  et al.
(2010)  Acs Chem.Biol.  5 : 377 - 392

PubMed: 20175559
DOI: 10.1021/cb900295g

Reaction

Chain : A
UniProt : Q40577 (5EAS_TOBAC)
Reaction: EC: Evidence:
Physiological Direction:
(2E,6E)-farnesyl diphosphate = (+)-5-epi-aristolochene + diphosphate 4.2.3.61 PubMed:16375847, Ref.3
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