Brand  (β version)

PDB ID: 3L5T

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Ligands
Code Name Style Show Link
956 1-methyl-2-oxo-4-[4-(thiophen-2-ylcarbonyl)piperazin-1-yl]-1,2-dihydroquinoline-3-carbonitrile PoSSuM
SO4 Sulfate ion PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 3L5T   PDBj   RCSB PDB   PDBe
Header : ISOMERASE
Title : Crystal structure of macrophage migration inhibitory factor (MIF) with thiophenepiperazinylquinolinone inhibitor at 1.86A resolution
Release Data : 2010-03-09
Compound :
mol_id molecule chains synonym
1 Macrophage migration inhibitory factor A,B,C MIF, Phenylpyruvate tautomerase, L-dopachrome tautomerase, L-dopachrome isomerase, Glycosylation-inhibiting factor, GIF
ec: 5.3.2.1, 5.3.3.12
Source :
mol_id organism_scientific organism_common expression_system
1 Homo sapiens  (taxid:9606) Human ESCHERICHIA COLI  (taxid:562)
gene: GLIF, MIF, MMIF
expression_system_strain: BL21(DE3)
expression_system_vector_type: PLASMID
expression_system_plasmid: PET22B
Authors : McLean, L., Zhang, Y.
Keywords : PROTEIN-LIGAND COMPLEX, CYTOKINE, CYTOPLASM, IMMUNE RESPONSE, INFLAMMATORY RESPONSE, INNATE IMMUNITY, ISOMERASE, PHOSPHOPROTEIN, SECRETED, Acetylation
Exp. method : X-RAY DIFFRACTION ( 1.860 Å )
Citation :

Fragment screening of inhibitors for MIF tautomerase reveals a cryptic surface binding site.

McLean, L.R.,Zhang, Y.,Li, H.  et al.
(2010)  Bioorg.Med.Chem.Lett.  20 : 1821 - 1824

PubMed: 20185308
DOI: 10.1016/j.bmcl.2010.02.009

Reaction

Chain : A, B, C
UniProt : P14174 (MIF_HUMAN)
Reaction: EC: Evidence:
Physiological Direction:
3-phenylpyruvate = enol-phenylpyruvate 5.3.2.1 PubMed:11439086
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L-dopachrome = 5,6-dihydroxyindole-2-carboxylate 5.3.3.12 PubMed:17526494
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