Brand  (β version)

PDB ID: 3K9Y

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Ligands
Code Name Style Show Link
HEM Protoporphyrin ix containing Fe PoSSuM
CM5 5-cyclohexyl-1-pentyl-beta-D-maltoside PoSSuM
Non-standard Residues
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Glycosylation
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Structure summary

Code : 3K9Y   PDBj   RCSB PDB   PDBe
Header : OXIDOREDUCTASE
Title : Crystal structure of rat mitochondrial P450 24A1 S57D in complex with CYMAL-5
Release Data : 2009-12-15
Compound :
mol_id molecule chains synonym
1 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial A,B Vitamin D(3) 24-hydroxylase, 24-OHase, Cytochrome P450 24A1, P450-CC24
ec: 1.14.13.-
fragment: residues 34-514
mutation: S57D
Source :
mol_id organism_scientific organism_common expression_system
1 Rattus norvegicus  (taxid:10116) Rat Escherichia coli  (taxid:562)
gene: Cyp24, Cyp24a1
expression_system_strain: DH5alpha-FIQ
expression_system_vector_type: Plasmid
expression_system_plasmid: pTRC99A
Authors : Annalora, A.J., Goodin, D.B., Hong, W., Zhang, Q., Johnson, E.F., Stout, C.D.
Keywords : MITOCHONDRIAL CYTOCHROME P450, MONOTOPIC MEMBRANE PROTEIN, MONOOXYGENASE, VITAMIN D HORMONE METABOLISM, ADRENODOXIN, OXIDOREDUCTASE
Exp. method : X-RAY DIFFRACTION ( 2.8 Å )
Citation :

Crystal structure of CYP24A1, a mitochondrial cytochrome P450 involved in vitamin D metabolism.

Annalora, A.J.,Goodin, D.B.,Hong, W.X.  et al.
(2010)  J.Mol.Biol.  396 : 441 - 451

PubMed: 19961857
DOI: 10.1016/j.jmb.2009.11.057

Reaction

Chain : A, B
UniProt : Q09128 (CP24A_RAT)
Reaction: EC: Evidence:
Physiological Direction:
calcitriol + 2 H(+) + O2 + 2 reduced [adrenodoxin] = calcitetrol + H2O + 2 oxidized [adrenodoxin] 1.14.15.16 PubMed:10231362, PubMed:16617161, PubMed:2026586
left-to-right PubMed:2026586
calcitetrol + 2 H(+) + O2 + 2 reduced [adrenodoxin] = (1S)- 1,25-dihydroxy-24-oxocalciol + 2 H2O + 2 oxidized [adrenodoxin] 1.14.15.16 PubMed:10231362, PubMed:16617161
left-to-right PubMed:16617161
(1S)-1,25-dihydroxy-24-oxocalciol + 2 H(+) + O2 + 2 reduced [adrenodoxin] = (1S)-1,23,25-trihydroxy-24-oxocalciol + H2O + 2 oxidized [adrenodoxin] 1.14.15.16 PubMed:10231362, PubMed:16617161
left-to-right PubMed:16617161
(1S)-1,23-dihydroxy-24,25,26,27-tetranorcalciol + 2 H(+) + O2 + 2 reduced [adrenodoxin] = (1S)-1-hydroxy-23-oxo-24,25,26,27- tetranorcalciol + 2 H2O + 2 oxidized [adrenodoxin] 1.14.15.16 PubMed:10231362
left-to-right
(1S)-1-hydroxy-23-oxo-24,25,26,27-tetranorcalciol + H(+) + O2 + 2 reduced [adrenodoxin] = calcitroate + H2O + 2 oxidized [adrenodoxin] 1.14.15.16 PubMed:10231362
left-to-right
calcidiol + 2 H(+) + O2 + 2 reduced [adrenodoxin] = H2O + 2 oxidized [adrenodoxin] + secalciferol 1.14.15.16 PubMed:10231362, PubMed:2026586
left-to-right PubMed:2026586
2 H(+) + O2 + 2 reduced [adrenodoxin] + secalciferol = 25- hydroxy-24-oxocalciol + 2 H2O + 2 oxidized [adrenodoxin] - PubMed:10231362
left-to-right
25-hydroxy-24-oxocalciol + 2 H(+) + O2 + 2 reduced [adrenodoxin] = 23S,25-dihydroxy-24-oxocholecalciferol + H2O + 2 oxidized [adrenodoxin] - PubMed:10231362
left-to-right
20S,23-dihydroxycholecalciferol + 2 H(+) + O2 + 2 reduced [adrenodoxin] = 20S,23,25-trihydroxycholecalciferol + H2O + 2 oxidized [adrenodoxin] - PubMed:25727742
left-to-right
20S,23-dihydroxycholecalciferol + 2 H(+) + O2 + 2 reduced [adrenodoxin] = 20S,23,24-trihydroxycholecalciferol + H2O + 2 oxidized [adrenodoxin] - PubMed:25727742
left-to-right
20S-hydroxycholecalciferol + 2 H(+) + O2 + 2 reduced [adrenodoxin] = 20S,25-dihydroxycholecalciferol + H2O + 2 oxidized [adrenodoxin] - PubMed:25727742
left-to-right
20S-hydroxycholecalciferol + 2 H(+) + O2 + 2 reduced [adrenodoxin] = 20S,24S-dihydroxycholecalciferol + H2O + 2 oxidized [adrenodoxin] - PubMed:25727742
left-to-right
20S-hydroxycholecalciferol + 2 H(+) + O2 + 2 reduced [adrenodoxin] = 20S,24R-dihydroxycholecalciferol + H2O + 2 oxidized [adrenodoxin] - PubMed:25727742
left-to-right